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Ghrelin and motilin are cosecreted from a prominent endocrine cell population in the small intestine

Wierup, Nils LU ; Björkqvist, Maria LU ; Weström, Björn LU ; Pierzynowski, Stefan LU ; Sundler, Frank LU and Sjölund, Kristina LU (2007) In Journal of Clinical Endocrinology and Metabolism 92(9). p.3573-3581
Abstract
Context: Ghrelin is a novel hormone produced mainly in the gastric body. Hitherto, mapping studies of ghrelin cells covering the entire gastrointestinal (GI) tract in humans have been lacking. Furthermore, the phenotype of extragastric ghrelin cells is not known. Objective: The objective of the study was to perform a detailed mapping with specimens from all parts of the GI tract, and colocalization studies to phenotype ghrelin cells along the tract. In addition, mapping of ghrelin cells was performed in porcine GI tract, and the plasma profiles of ghrelin and motilin in blood from the porcine intestine were measured. Design: Biopsies from patients were obtained during gastroscopy or surgery. Ghrelin cell density and phenotyping was... (More)
Context: Ghrelin is a novel hormone produced mainly in the gastric body. Hitherto, mapping studies of ghrelin cells covering the entire gastrointestinal (GI) tract in humans have been lacking. Furthermore, the phenotype of extragastric ghrelin cells is not known. Objective: The objective of the study was to perform a detailed mapping with specimens from all parts of the GI tract, and colocalization studies to phenotype ghrelin cells along the tract. In addition, mapping of ghrelin cells was performed in porcine GI tract, and the plasma profiles of ghrelin and motilin in blood from the porcine intestine were measured. Design: Biopsies from patients were obtained during gastroscopy or surgery. Ghrelin cell density and phenotyping was assessed with immunocytochemistry, in situ hybridization, and immunogold electron microscopy. Plasma ghrelin and motilin levels were measured in pigs, fitted with cannulas in the mesenteric vein. Results: The upper small intestine is unexpectedly rich in ghrelin cells, and these cells contribute to circulating ghrelin. Ghrelin and motilin are coproduced in the same cells in the duodenum and jejunum of both species, and ghrelin and motilin are stored in all secretory granules of such cells in humans, indicating cosecretion. The plasma profiles of ghrelin and motilin in pig were parallel, and a correlation between ghrelin and motilin ( r(2) = 0.22; P < 0.001) was evident in intestinal blood. Conclusions: The upper small intestine is an important source of ghrelin. The likely cosecretion of intestinal ghrelin and motilin suggests concerted actions of the two hormones. These data may have implications for understanding gut motility and clinical implications for dysmotility and bariatric surgery. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ghrelin • motilin • human • porcine • gastro-intestinal tract • gut hormones • co-localisation • co-secretion
in
Journal of Clinical Endocrinology and Metabolism
volume
92
issue
9
pages
3573 - 3581
publisher
The Endocrine Society
external identifiers
  • wos:000249279800037
  • scopus:34548777165
ISSN
1945-7197
DOI
10.1210/jc.2006-2756
language
English
LU publication?
yes
id
27d7a12b-a2f3-401d-a7c1-a3bbf07e24d1 (old id 687952)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17595255&dopt=Abstract
date added to LUP
2007-12-19 11:28:34
date last changed
2017-08-27 05:12:10
@article{27d7a12b-a2f3-401d-a7c1-a3bbf07e24d1,
  abstract     = {Context: Ghrelin is a novel hormone produced mainly in the gastric body. Hitherto, mapping studies of ghrelin cells covering the entire gastrointestinal (GI) tract in humans have been lacking. Furthermore, the phenotype of extragastric ghrelin cells is not known. Objective: The objective of the study was to perform a detailed mapping with specimens from all parts of the GI tract, and colocalization studies to phenotype ghrelin cells along the tract. In addition, mapping of ghrelin cells was performed in porcine GI tract, and the plasma profiles of ghrelin and motilin in blood from the porcine intestine were measured. Design: Biopsies from patients were obtained during gastroscopy or surgery. Ghrelin cell density and phenotyping was assessed with immunocytochemistry, in situ hybridization, and immunogold electron microscopy. Plasma ghrelin and motilin levels were measured in pigs, fitted with cannulas in the mesenteric vein. Results: The upper small intestine is unexpectedly rich in ghrelin cells, and these cells contribute to circulating ghrelin. Ghrelin and motilin are coproduced in the same cells in the duodenum and jejunum of both species, and ghrelin and motilin are stored in all secretory granules of such cells in humans, indicating cosecretion. The plasma profiles of ghrelin and motilin in pig were parallel, and a correlation between ghrelin and motilin ( r(2) = 0.22; P &lt; 0.001) was evident in intestinal blood. Conclusions: The upper small intestine is an important source of ghrelin. The likely cosecretion of intestinal ghrelin and motilin suggests concerted actions of the two hormones. These data may have implications for understanding gut motility and clinical implications for dysmotility and bariatric surgery.},
  author       = {Wierup, Nils and Björkqvist, Maria and Weström, Björn and Pierzynowski, Stefan and Sundler, Frank and Sjölund, Kristina},
  issn         = {1945-7197},
  keyword      = {ghrelin • motilin • human • porcine • gastro-intestinal tract • gut hormones • co-localisation • co-secretion},
  language     = {eng},
  number       = {9},
  pages        = {3573--3581},
  publisher    = {The Endocrine Society},
  series       = {Journal of Clinical Endocrinology and Metabolism},
  title        = {Ghrelin and motilin are cosecreted from a prominent endocrine cell population in the small intestine},
  url          = {http://dx.doi.org/10.1210/jc.2006-2756},
  volume       = {92},
  year         = {2007},
}