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The effect of reversible permeabilization and post-electroporation resting on the survival of Thai basil (O. Basilicum cv. thyrsiflora) leaves during drying

Thamkaew, Grant LU ; Wadsö, Lars LU ; Rasmusson, Allan G. LU and Gómez Galindo, Federico LU (2021) In Bioelectrochemistry 142.
Abstract

Horticultural crops have a low tolerance to dehydration. In this paper, we show that the reversible electroporation (200 monopolar, rectangular pulses of 50 µs pulse duration, 760 µs between pulses and nominal field strength of 650 V/cm) of Thai basil leaves followed by 24 h resting before hot air drying at 40 °C enhanced the survivability of the tissues at certain levels of dehydration (moisture ratio = 0.2 and 0.1). However, this increased survival was rather limited. Through measurements of metabolic heat production during resting, rehydration kinetics, respiration and photosynthesis of the rehydrated leaves, we show that resting after the application of a reversible pulse-electric field (PEF) may allow a phase of hardening that has... (More)

Horticultural crops have a low tolerance to dehydration. In this paper, we show that the reversible electroporation (200 monopolar, rectangular pulses of 50 µs pulse duration, 760 µs between pulses and nominal field strength of 650 V/cm) of Thai basil leaves followed by 24 h resting before hot air drying at 40 °C enhanced the survivability of the tissues at certain levels of dehydration (moisture ratio = 0.2 and 0.1). However, this increased survival was rather limited. Through measurements of metabolic heat production during resting, rehydration kinetics, respiration and photosynthesis of the rehydrated leaves, we show that resting after the application of a reversible pulse-electric field (PEF) may allow a phase of hardening that has a protective effect on the cells, thus decreasing damage during the subsequent drying phase. Increased preservation of cell vitality would be associated with a more turgid and fresh-like rehydrated product, as cells would have the capacity to retain the rehydration water.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Ion leakage, Pulsed electric field, Respiration, Stress responses, Viability preservation
in
Bioelectrochemistry
volume
142
article number
107912
publisher
Elsevier
external identifiers
  • pmid:34358981
  • scopus:85111701563
ISSN
1567-5394
DOI
10.1016/j.bioelechem.2021.107912
project
Advancing optical microscopy for materials engineering in Lund University
language
English
LU publication?
yes
id
7086a74b-4489-415d-b39f-77e7c5a697bb
date added to LUP
2021-12-22 10:36:06
date last changed
2024-03-23 16:14:04
@article{7086a74b-4489-415d-b39f-77e7c5a697bb,
  abstract     = {{<p>Horticultural crops have a low tolerance to dehydration. In this paper, we show that the reversible electroporation (200 monopolar, rectangular pulses of 50 µs pulse duration, 760 µs between pulses and nominal field strength of 650 V/cm) of Thai basil leaves followed by 24 h resting before hot air drying at 40 °C enhanced the survivability of the tissues at certain levels of dehydration (moisture ratio = 0.2 and 0.1). However, this increased survival was rather limited. Through measurements of metabolic heat production during resting, rehydration kinetics, respiration and photosynthesis of the rehydrated leaves, we show that resting after the application of a reversible pulse-electric field (PEF) may allow a phase of hardening that has a protective effect on the cells, thus decreasing damage during the subsequent drying phase. Increased preservation of cell vitality would be associated with a more turgid and fresh-like rehydrated product, as cells would have the capacity to retain the rehydration water.</p>}},
  author       = {{Thamkaew, Grant and Wadsö, Lars and Rasmusson, Allan G. and Gómez Galindo, Federico}},
  issn         = {{1567-5394}},
  keywords     = {{Ion leakage; Pulsed electric field; Respiration; Stress responses; Viability preservation}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Bioelectrochemistry}},
  title        = {{The effect of reversible permeabilization and post-electroporation resting on the survival of Thai basil (O. Basilicum cv. thyrsiflora) leaves during drying}},
  url          = {{http://dx.doi.org/10.1016/j.bioelechem.2021.107912}},
  doi          = {{10.1016/j.bioelechem.2021.107912}},
  volume       = {{142}},
  year         = {{2021}},
}