Isotope dilution ESI-LC-MS/MS for quantification of free and total Nε-(1-Carboxymethyl)-l-Lysine and free Nε-(1-Carboxyethyl)-l-Lysine: Comparison of total Nε-(1-Carboxymethyl)-l-Lysine levels measured with new method to ELISA assay in gruel samples.
(2013) In Food Chemistry 141(4). p.4253-4259- Abstract
- ESI-LC-MS/MS method with isotope dilution and SPE based on cation-exchange was developed for determination of free and total Nε-(1-Carboxymethyl)-l-Lysine (CML) and free Nε-(1-Carboxyethyl)-l-Lysine (CEL). The use of nonafluoropentanoic acid in mobile phase was omitted, SPE recoveries of 82±3% and 91±10% (n=6) for CML and CEL respectively and, calibration curves (R(2)>0.9985) were attained. The method was applied to gruel samples and LoQ for the method was 5ng/ml, RSD <10% and accuracy was 115%. Total CML levels in the gruel samples varied from 103-408mg/kg protein. Free CML levels which were 1000 times lower than total CML were three times higher than free CEL levels. CML in a gruel sample was 127±7, 84±9 and 253±28mg/kg using the... (More)
- ESI-LC-MS/MS method with isotope dilution and SPE based on cation-exchange was developed for determination of free and total Nε-(1-Carboxymethyl)-l-Lysine (CML) and free Nε-(1-Carboxyethyl)-l-Lysine (CEL). The use of nonafluoropentanoic acid in mobile phase was omitted, SPE recoveries of 82±3% and 91±10% (n=6) for CML and CEL respectively and, calibration curves (R(2)>0.9985) were attained. The method was applied to gruel samples and LoQ for the method was 5ng/ml, RSD <10% and accuracy was 115%. Total CML levels in the gruel samples varied from 103-408mg/kg protein. Free CML levels which were 1000 times lower than total CML were three times higher than free CEL levels. CML in a gruel sample was 127±7, 84±9 and 253±28mg/kg using the current ESI-LC-MS/MS, ELISA and GC-MS respectively. The described method has advantages over ELISA with respect to reproducibility and specificity and over GC-MS with respect to reproducibility. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4066373
- author
- Tareke, Eden
LU
; Forslund, Anna
LU
; Lindh, Christian
LU
; Fahlgren, Christer LU and Östman, Elin LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Food Chemistry
- volume
- 141
- issue
- 4
- pages
- 4253 - 4259
- publisher
- Elsevier
- external identifiers
-
- wos:000324848000131
- pmid:23993613
- scopus:84884611476
- pmid:23993613
- ISSN
- 1873-7072
- DOI
- 10.1016/j.foodchem.2013.07.003
- project
- ANTIDIABETIC FOOD CENTRE
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Occupational and Environmental Medicine (013078001), Applied Nutrition and Food Chemistry (011001300)
- id
- 71e3ae42-43c8-48c4-b7f7-d83eb190b559 (old id 4066373)
- date added to LUP
- 2016-04-01 14:21:47
- date last changed
- 2023-12-12 04:16:11
@article{71e3ae42-43c8-48c4-b7f7-d83eb190b559, abstract = {{ESI-LC-MS/MS method with isotope dilution and SPE based on cation-exchange was developed for determination of free and total Nε-(1-Carboxymethyl)-l-Lysine (CML) and free Nε-(1-Carboxyethyl)-l-Lysine (CEL). The use of nonafluoropentanoic acid in mobile phase was omitted, SPE recoveries of 82±3% and 91±10% (n=6) for CML and CEL respectively and, calibration curves (R(2)>0.9985) were attained. The method was applied to gruel samples and LoQ for the method was 5ng/ml, RSD <10% and accuracy was 115%. Total CML levels in the gruel samples varied from 103-408mg/kg protein. Free CML levels which were 1000 times lower than total CML were three times higher than free CEL levels. CML in a gruel sample was 127±7, 84±9 and 253±28mg/kg using the current ESI-LC-MS/MS, ELISA and GC-MS respectively. The described method has advantages over ELISA with respect to reproducibility and specificity and over GC-MS with respect to reproducibility.}}, author = {{Tareke, Eden and Forslund, Anna and Lindh, Christian and Fahlgren, Christer and Östman, Elin}}, issn = {{1873-7072}}, language = {{eng}}, number = {{4}}, pages = {{4253--4259}}, publisher = {{Elsevier}}, series = {{Food Chemistry}}, title = {{Isotope dilution ESI-LC-MS/MS for quantification of free and total Nε-(1-Carboxymethyl)-l-Lysine and free Nε-(1-Carboxyethyl)-l-Lysine: Comparison of total Nε-(1-Carboxymethyl)-l-Lysine levels measured with new method to ELISA assay in gruel samples.}}, url = {{http://dx.doi.org/10.1016/j.foodchem.2013.07.003}}, doi = {{10.1016/j.foodchem.2013.07.003}}, volume = {{141}}, year = {{2013}}, }