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Isotope dilution ESI-LC-MS/MS for quantification of free and total Nε-(1-Carboxymethyl)-l-Lysine and free Nε-(1-Carboxyethyl)-l-Lysine: Comparison of total Nε-(1-Carboxymethyl)-l-Lysine levels measured with new method to ELISA assay in gruel samples.

Tareke, Eden LU ; Forslund, Anna LU ; Lindh, Christian LU orcid ; Fahlgren, Christer LU and Östman, Elin LU (2013) In Food Chemistry 141(4). p.4253-4259
Abstract
ESI-LC-MS/MS method with isotope dilution and SPE based on cation-exchange was developed for determination of free and total Nε-(1-Carboxymethyl)-l-Lysine (CML) and free Nε-(1-Carboxyethyl)-l-Lysine (CEL). The use of nonafluoropentanoic acid in mobile phase was omitted, SPE recoveries of 82±3% and 91±10% (n=6) for CML and CEL respectively and, calibration curves (R(2)>0.9985) were attained. The method was applied to gruel samples and LoQ for the method was 5ng/ml, RSD <10% and accuracy was 115%. Total CML levels in the gruel samples varied from 103-408mg/kg protein. Free CML levels which were 1000 times lower than total CML were three times higher than free CEL levels. CML in a gruel sample was 127±7, 84±9 and 253±28mg/kg using the... (More)
ESI-LC-MS/MS method with isotope dilution and SPE based on cation-exchange was developed for determination of free and total Nε-(1-Carboxymethyl)-l-Lysine (CML) and free Nε-(1-Carboxyethyl)-l-Lysine (CEL). The use of nonafluoropentanoic acid in mobile phase was omitted, SPE recoveries of 82±3% and 91±10% (n=6) for CML and CEL respectively and, calibration curves (R(2)>0.9985) were attained. The method was applied to gruel samples and LoQ for the method was 5ng/ml, RSD <10% and accuracy was 115%. Total CML levels in the gruel samples varied from 103-408mg/kg protein. Free CML levels which were 1000 times lower than total CML were three times higher than free CEL levels. CML in a gruel sample was 127±7, 84±9 and 253±28mg/kg using the current ESI-LC-MS/MS, ELISA and GC-MS respectively. The described method has advantages over ELISA with respect to reproducibility and specificity and over GC-MS with respect to reproducibility. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Food Chemistry
volume
141
issue
4
pages
4253 - 4259
publisher
Elsevier
external identifiers
  • wos:000324848000131
  • pmid:23993613
  • scopus:84884611476
  • pmid:23993613
ISSN
1873-7072
DOI
10.1016/j.foodchem.2013.07.003
project
ANTIDIABETIC FOOD CENTRE
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Occupational and Environmental Medicine (013078001), Applied Nutrition and Food Chemistry (011001300)
id
71e3ae42-43c8-48c4-b7f7-d83eb190b559 (old id 4066373)
date added to LUP
2016-04-01 14:21:47
date last changed
2023-12-12 04:16:11
@article{71e3ae42-43c8-48c4-b7f7-d83eb190b559,
  abstract     = {{ESI-LC-MS/MS method with isotope dilution and SPE based on cation-exchange was developed for determination of free and total Nε-(1-Carboxymethyl)-l-Lysine (CML) and free Nε-(1-Carboxyethyl)-l-Lysine (CEL). The use of nonafluoropentanoic acid in mobile phase was omitted, SPE recoveries of 82±3% and 91±10% (n=6) for CML and CEL respectively and, calibration curves (R(2)&gt;0.9985) were attained. The method was applied to gruel samples and LoQ for the method was 5ng/ml, RSD &lt;10% and accuracy was 115%. Total CML levels in the gruel samples varied from 103-408mg/kg protein. Free CML levels which were 1000 times lower than total CML were three times higher than free CEL levels. CML in a gruel sample was 127±7, 84±9 and 253±28mg/kg using the current ESI-LC-MS/MS, ELISA and GC-MS respectively. The described method has advantages over ELISA with respect to reproducibility and specificity and over GC-MS with respect to reproducibility.}},
  author       = {{Tareke, Eden and Forslund, Anna and Lindh, Christian and Fahlgren, Christer and Östman, Elin}},
  issn         = {{1873-7072}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{4253--4259}},
  publisher    = {{Elsevier}},
  series       = {{Food Chemistry}},
  title        = {{Isotope dilution ESI-LC-MS/MS for quantification of free and total Nε-(1-Carboxymethyl)-l-Lysine and free Nε-(1-Carboxyethyl)-l-Lysine: Comparison of total Nε-(1-Carboxymethyl)-l-Lysine levels measured with new method to ELISA assay in gruel samples.}},
  url          = {{http://dx.doi.org/10.1016/j.foodchem.2013.07.003}},
  doi          = {{10.1016/j.foodchem.2013.07.003}},
  volume       = {{141}},
  year         = {{2013}},
}