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Visualization and genetic modification of resident brain microglia using lentiviral vectors regulated by microRNA-9.

Åkerblom, Malin LU ; Sachdeva, Rohit LU ; Quintino, Luis LU ; Wettergren, Erika Elgstrand ; Chapman, Katie LU ; Manfre, Giuseppe LU ; Lindvall, Olle LU ; Lundberg, Cecilia LU and Jakobsson, Johan LU (2013) In Nature Communications 4.
Abstract
Functional studies of resident microglia require molecular tools for their genetic manipulation. Here we show that microRNA-9-regulated lentiviral vectors can be used for the targeted genetic modification of resident microglia in the rodent brain. Using transgenic reporter mice, we demonstrate that murine microglia lack microRNA-9 activity, whereas most other cells in the brain express microRNA-9. Injection of microRNA-9-regulated vectors into the adult rat brain induces transgene expression specifically in cells with morphological features typical of ramified microglia. The majority of transgene-expressing cells colabels with the microglia marker Iba1. We use this approach to visualize and isolate activated resident microglia without... (More)
Functional studies of resident microglia require molecular tools for their genetic manipulation. Here we show that microRNA-9-regulated lentiviral vectors can be used for the targeted genetic modification of resident microglia in the rodent brain. Using transgenic reporter mice, we demonstrate that murine microglia lack microRNA-9 activity, whereas most other cells in the brain express microRNA-9. Injection of microRNA-9-regulated vectors into the adult rat brain induces transgene expression specifically in cells with morphological features typical of ramified microglia. The majority of transgene-expressing cells colabels with the microglia marker Iba1. We use this approach to visualize and isolate activated resident microglia without affecting circulating and infiltrating monocytes or macrophages in an excitotoxic lesion model in rat striatum. The microRNA-9-regulated vectors described here are a straightforward and powerful tool that facilitates functional studies of resident microglia. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nature Communications
volume
4
article number
1770
publisher
Nature Publishing Group
external identifiers
  • wos:000318872100127
  • pmid:23612311
  • scopus:84877730401
  • pmid:23612311
ISSN
2041-1723
DOI
10.1038/ncomms2801
language
English
LU publication?
yes
id
7c6f049e-7237-420f-aba8-3c2a582351d7 (old id 3733413)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23612311?dopt=Abstract
date added to LUP
2016-04-01 14:29:34
date last changed
2020-10-07 03:49:16
@article{7c6f049e-7237-420f-aba8-3c2a582351d7,
  abstract     = {Functional studies of resident microglia require molecular tools for their genetic manipulation. Here we show that microRNA-9-regulated lentiviral vectors can be used for the targeted genetic modification of resident microglia in the rodent brain. Using transgenic reporter mice, we demonstrate that murine microglia lack microRNA-9 activity, whereas most other cells in the brain express microRNA-9. Injection of microRNA-9-regulated vectors into the adult rat brain induces transgene expression specifically in cells with morphological features typical of ramified microglia. The majority of transgene-expressing cells colabels with the microglia marker Iba1. We use this approach to visualize and isolate activated resident microglia without affecting circulating and infiltrating monocytes or macrophages in an excitotoxic lesion model in rat striatum. The microRNA-9-regulated vectors described here are a straightforward and powerful tool that facilitates functional studies of resident microglia.},
  author       = {Åkerblom, Malin and Sachdeva, Rohit and Quintino, Luis and Wettergren, Erika Elgstrand and Chapman, Katie and Manfre, Giuseppe and Lindvall, Olle and Lundberg, Cecilia and Jakobsson, Johan},
  issn         = {2041-1723},
  language     = {eng},
  publisher    = {Nature Publishing Group},
  series       = {Nature Communications},
  title        = {Visualization and genetic modification of resident brain microglia using lentiviral vectors regulated by microRNA-9.},
  url          = {http://dx.doi.org/10.1038/ncomms2801},
  doi          = {10.1038/ncomms2801},
  volume       = {4},
  year         = {2013},
}