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Intestinal bacteria and permeability during experimental acute pancreatitis in rats

Axelsson, Jakob B LU ; Eckerwall, Gunilla LU ; Norrman, Gustav; Dib, Marwan LU ; Nehéz, Laszlo LU ; Soltesz, Vasile LU ; Weström, Björn LU and Andersson, Roland LU (2006) In Annals of Gastroenterology 19(3). p.276-284
Abstract
Background: An increase in intestinal permeability and subsequent bacterial translocation has been demonstrated in

critical illness. Cellulose derivatives have in the past been shown to reduce gut leakage following liver resection.



Aims: The aim of the present study was to evaluate changes

in microbial counts in experimental acute pancreatitis and

the effect of pre-treatment with cellulose derivatives and

N-acetyl cysteine.



Subjects: 92 male Sprague Dawley rats.

Methods: Acute pancreatitis was induced by intraductal taurodeoxycholic acid infusion. Animals received oral pretreatment and were randomized to either sham operation

or the pancreatitis... (More)
Background: An increase in intestinal permeability and subsequent bacterial translocation has been demonstrated in

critical illness. Cellulose derivatives have in the past been shown to reduce gut leakage following liver resection.



Aims: The aim of the present study was to evaluate changes

in microbial counts in experimental acute pancreatitis and

the effect of pre-treatment with cellulose derivatives and

N-acetyl cysteine.



Subjects: 92 male Sprague Dawley rats.

Methods: Acute pancreatitis was induced by intraductal taurodeoxycholic acid infusion. Animals received oral pretreatment and were randomized to either sham operation

or the pancreatitis groups, with or without pre-treatment

with cellulose derivatives, the antioxidant or their combinations. Intestinal bacterial populations and permeability were evaluated using bacterial counts and Ussing chamber, respectively.



Results: The number of E. coli increased in the luminal content and ileal and colonic mucosa, but levels were restored to almost those seen in controls in all pre-treatment groups except for N-acetyl cysteine. When intestinal permeability was measured, none of the treatment groups showed significant differences compared to challenge, except for Nacetyl cysteine, which significantly increased permeability.



Conclusion: Pre-treatment with cellulose derivatives was

more efficient against disturbances in intestinal permeability and microbial populations than the antioxidant Nacetyl cysteine. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
dietary fibres, acute pancreatitis, bacterial counts, intestinal permeability
in
Annals of Gastroenterology
volume
19
issue
3
pages
276 - 284
publisher
Hellenic Society of Gastroenterology
external identifiers
  • scopus:60249089602
ISSN
1108-7471
language
English
LU publication?
yes
id
85a28d74-3da3-4433-9dfb-7798d228a689 (old id 1137017)
alternative location
http://www.annalsgastro.gr/index.php/annalsgastro/article/download/463/390
date added to LUP
2008-05-22 10:48:35
date last changed
2019-02-20 08:00:47
@article{85a28d74-3da3-4433-9dfb-7798d228a689,
  abstract     = {Background: An increase in intestinal permeability and subsequent bacterial translocation has been demonstrated in<br/><br>
critical illness. Cellulose derivatives have in the past been shown to reduce gut leakage following liver resection.<br/><br>
<br/><br>
Aims: The aim of the present study was to evaluate changes<br/><br>
in microbial counts in experimental acute pancreatitis and<br/><br>
the effect of pre-treatment with cellulose derivatives and<br/><br>
N-acetyl cysteine. <br/><br>
<br/><br>
Subjects: 92 male Sprague Dawley rats.<br/><br>
Methods: Acute pancreatitis was induced by intraductal taurodeoxycholic acid infusion. Animals received oral pretreatment and were randomized to either sham operation<br/><br>
or the pancreatitis groups, with or without pre-treatment<br/><br>
with cellulose derivatives, the antioxidant or their combinations. Intestinal bacterial populations and permeability were evaluated using bacterial counts and Ussing chamber, respectively.<br/><br>
<br/><br>
Results: The number of E. coli increased in the luminal content and ileal and colonic mucosa, but levels were restored to almost those seen in controls in all pre-treatment groups except for N-acetyl cysteine. When intestinal permeability was measured, none of the treatment groups showed significant differences compared to challenge, except for Nacetyl cysteine, which significantly increased permeability.<br/><br>
<br/><br>
Conclusion: Pre-treatment with cellulose derivatives was<br/><br>
more efficient against disturbances in intestinal permeability and microbial populations than the antioxidant Nacetyl cysteine.},
  author       = {Axelsson, Jakob B and Eckerwall, Gunilla and Norrman, Gustav and Dib, Marwan and Nehéz, Laszlo and Soltesz, Vasile and Weström, Björn and Andersson, Roland},
  issn         = {1108-7471},
  keyword      = {dietary fibres,acute pancreatitis,bacterial counts,intestinal permeability},
  language     = {eng},
  number       = {3},
  pages        = {276--284},
  publisher    = {Hellenic Society of Gastroenterology},
  series       = {Annals of Gastroenterology},
  title        = {Intestinal bacteria and permeability during experimental acute pancreatitis in rats},
  volume       = {19},
  year         = {2006},
}