Characterization of high Arabinoxylan oat lines identified from a mutagenized oat population
(2023) In Food Chemistry 404.- Abstract
Oat (Avena sativa) is a nutritionally important cereal crop that is rich in health-promoting dietary fibers, favorable proteins and polar lipids. In this work, ca. 500 random lines of a mutagenized oat population of high genetic variation were screened for arabinoxylan (AX) content. This identified lines with up to 60% higher AX levels in flour from whole seed and up to 100% higher in flour from dehulled seeds, as compared to the original Belinda variety. In addition, the cellular localization of AX was determined in cross-sections of dehulled seeds from three high and one low AX line using a xylan-specific antibody. This revealed variations in the amount and localization of AX between high and low AX lines. The high AX lines will now... (More)
Oat (Avena sativa) is a nutritionally important cereal crop that is rich in health-promoting dietary fibers, favorable proteins and polar lipids. In this work, ca. 500 random lines of a mutagenized oat population of high genetic variation were screened for arabinoxylan (AX) content. This identified lines with up to 60% higher AX levels in flour from whole seed and up to 100% higher in flour from dehulled seeds, as compared to the original Belinda variety. In addition, the cellular localization of AX was determined in cross-sections of dehulled seeds from three high and one low AX line using a xylan-specific antibody. This revealed variations in the amount and localization of AX between high and low AX lines. The high AX lines will now serve as a starting point in the development of oat varieties with superior health-promoting and rheological properties.
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- author
- Alfredo Zambrano, José LU ; Thyagarajan, Aishwarya ; Sardari, Roya R.R. LU and Olsson, Olof LU
- organization
- publishing date
- 2023-03-15
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Arabinoxylan (AX), Fibers, High oat AX lines, Immunolocalization, Mutagenesis, Oat
- in
- Food Chemistry
- volume
- 404
- article number
- 134687
- publisher
- Elsevier
- external identifiers
-
- scopus:85140799738
- pmid:36323030
- ISSN
- 0308-8146
- DOI
- 10.1016/j.foodchem.2022.134687
- language
- English
- LU publication?
- yes
- id
- 8c5b7be1-700f-4f62-9b48-4c6c808508e7
- date added to LUP
- 2023-01-23 11:06:24
- date last changed
- 2024-06-11 17:57:46
@article{8c5b7be1-700f-4f62-9b48-4c6c808508e7,
abstract = {{<p>Oat (Avena sativa) is a nutritionally important cereal crop that is rich in health-promoting dietary fibers, favorable proteins and polar lipids. In this work, ca. 500 random lines of a mutagenized oat population of high genetic variation were screened for arabinoxylan (AX) content. This identified lines with up to 60% higher AX levels in flour from whole seed and up to 100% higher in flour from dehulled seeds, as compared to the original Belinda variety. In addition, the cellular localization of AX was determined in cross-sections of dehulled seeds from three high and one low AX line using a xylan-specific antibody. This revealed variations in the amount and localization of AX between high and low AX lines. The high AX lines will now serve as a starting point in the development of oat varieties with superior health-promoting and rheological properties.</p>}},
author = {{Alfredo Zambrano, José and Thyagarajan, Aishwarya and Sardari, Roya R.R. and Olsson, Olof}},
issn = {{0308-8146}},
keywords = {{Arabinoxylan (AX); Fibers; High oat AX lines; Immunolocalization; Mutagenesis; Oat}},
language = {{eng}},
month = {{03}},
publisher = {{Elsevier}},
series = {{Food Chemistry}},
title = {{Characterization of high Arabinoxylan oat lines identified from a mutagenized oat population}},
url = {{http://dx.doi.org/10.1016/j.foodchem.2022.134687}},
doi = {{10.1016/j.foodchem.2022.134687}},
volume = {{404}},
year = {{2023}},
}