Abundance of gut Prevotella at baseline and metabolic response to barley prebiotics
Sandberg, Jonna LU ; Kovatcheva-Datchary, Petia ; Björck, Inger LU ; Bäckhed, Fredrik and Nilsson, Anne LU
(2019)
In European Journal of Nutrition
58(6).
p.2365-2376
- Abstract
Purpose: We previously showed that short-term intervention with barley kernel bread (BKB) improved glucose tolerance. However, glucose tolerance was not improved in a subset of individuals (non-responders) who were characterized by a low Prevotella/Bacteroides ratio. The purpose of the present study was to investigate if the baseline Prevotella/Bacteroides ratio can be used to stratify metabolic responders and non-responders to barley dietary fiber (DF). Methods: Fecal samples were collected from 99 healthy humans with BMI < 28 kg/m2 between 50 and 70 years old. The abundance of fecal Prevotella and Bacteroides was quantified with 16S rRNA quantitative PCR. 33 subjects were grouped in three groups: subjects with highest... (More)
Purpose: We previously showed that short-term intervention with barley kernel bread (BKB) improved glucose tolerance. However, glucose tolerance was not improved in a subset of individuals (non-responders) who were characterized by a low Prevotella/Bacteroides ratio. The purpose of the present study was to investigate if the baseline Prevotella/Bacteroides ratio can be used to stratify metabolic responders and non-responders to barley dietary fiber (DF). Methods: Fecal samples were collected from 99 healthy humans with BMI < 28 kg/m2 between 50 and 70 years old. The abundance of fecal Prevotella and Bacteroides was quantified with 16S rRNA quantitative PCR. 33 subjects were grouped in three groups: subjects with highest Prevotella/Bacteroides ratios, “HP”, n = 12; subjects with lowest Prevotella/Bacteroides ratios, “LP”, n = 13; and subjects with high abundance of both measured bacteria, HPB, n = 8. A 3-day randomized crossover intervention with BKB and white wheat bread (control) was performed. Cardiometabolic test variables were analyzed the next day following a standardized breakfast. Results: The BKB intervention lowered the blood glucose responses to the breakfast independently of Prevotella/Bacteroides ratios (P < 0.01). However, independently of intervention, the HP group displayed an overall lower insulin response and lower IL-6 concentrations compared with the LP group (P < 0.05). Furthermore, the groups HP and HPB showed lower hunger sensations compared to the LP group (P < 0.05). Conclusions: Here we show that the abundance of gut Prevotella and Bacteroides at baseline did not stratify metabolic responders and non-responders to barley DF intervention. However, our results indicate the importance of gut microbiota in host metabolic regulation, further suggesting that higher Prevotella/Bacteroides ratio may be favorable. ClinicalTrials.gov ID: NCT02427555
(Less)
- author
- Sandberg, Jonna
LU
; Kovatcheva-Datchary, Petia
; Björck, Inger
LU
; Bäckhed, Fredrik
and Nilsson, Anne
LU
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bacteroides, Barley, Glucose regulation, Prevention, Prevotella, Stratification
- in
- European Journal of Nutrition
- volume
- 58
- issue
- 6
- pages
- 2365 - 2376
- publisher
- Springer
- external identifiers
-
- pmid:30046942
- scopus:85050738382
- ISSN
- 1436-6207
- DOI
- 10.1007/s00394-018-1788-9
- language
- English
- LU publication?
- yes
- id
- ac65af2d-5207-42ce-813f-e23dfd1a0223
- date added to LUP
- 2018-10-01 09:02:34
- date last changed
- 2024-07-08 19:02:26
@article{ac65af2d-5207-42ce-813f-e23dfd1a0223,
abstract = {{<p>Purpose: We previously showed that short-term intervention with barley kernel bread (BKB) improved glucose tolerance. However, glucose tolerance was not improved in a subset of individuals (non-responders) who were characterized by a low Prevotella/Bacteroides ratio. The purpose of the present study was to investigate if the baseline Prevotella/Bacteroides ratio can be used to stratify metabolic responders and non-responders to barley dietary fiber (DF). Methods: Fecal samples were collected from 99 healthy humans with BMI < 28 kg/m<sup>2</sup> between 50 and 70 years old. The abundance of fecal Prevotella and Bacteroides was quantified with 16S rRNA quantitative PCR. 33 subjects were grouped in three groups: subjects with highest Prevotella/Bacteroides ratios, “HP”, n = 12; subjects with lowest Prevotella/Bacteroides ratios, “LP”, n = 13; and subjects with high abundance of both measured bacteria, HPB, n = 8. A 3-day randomized crossover intervention with BKB and white wheat bread (control) was performed. Cardiometabolic test variables were analyzed the next day following a standardized breakfast. Results: The BKB intervention lowered the blood glucose responses to the breakfast independently of Prevotella/Bacteroides ratios (P < 0.01). However, independently of intervention, the HP group displayed an overall lower insulin response and lower IL-6 concentrations compared with the LP group (P < 0.05). Furthermore, the groups HP and HPB showed lower hunger sensations compared to the LP group (P < 0.05). Conclusions: Here we show that the abundance of gut Prevotella and Bacteroides at baseline did not stratify metabolic responders and non-responders to barley DF intervention. However, our results indicate the importance of gut microbiota in host metabolic regulation, further suggesting that higher Prevotella/Bacteroides ratio may be favorable. ClinicalTrials.gov ID: NCT02427555</p>}},
author = {{Sandberg, Jonna and Kovatcheva-Datchary, Petia and Björck, Inger and Bäckhed, Fredrik and Nilsson, Anne}},
issn = {{1436-6207}},
keywords = {{Bacteroides; Barley; Glucose regulation; Prevention; Prevotella; Stratification}},
language = {{eng}},
number = {{6}},
pages = {{2365--2376}},
publisher = {{Springer}},
series = {{European Journal of Nutrition}},
title = {{Abundance of gut Prevotella at baseline and metabolic response to barley prebiotics}},
url = {{http://dx.doi.org/10.1007/s00394-018-1788-9}},
doi = {{10.1007/s00394-018-1788-9}},
volume = {{58}},
year = {{2019}},
}