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Monobutyrin Reduces Liver Cholesterol and Improves Intestinal Barrier Function in Rats Fed High-Fat Diets

Nguyen, Thao Duy LU ; Prykhodko, Olena LU ; Hållenius, Frida F. LU and Nyman, Margareta LU (2019) In Nutrients 11(2).
Abstract

Butyric acid has been shown to reduce high-fat diet-related metabolic disturbances and to improve intestinal barrier function due to its potent anti-inflammatory capacity. This study investigates whether a butyric acid ester, monobutyrin (MB) affects lipid profiles and gut barrier function in a dose-response manner in rats fed butter- or lard-based high-fat diets. Four-week-old male Wistar rats were fed butter-based diets containing 0, 0.25, 0.75 and 1.5 MB g/100 g (dry weight basis) or 0.5 glycerol g/100 g, and diets with lard (La) containing 0 and 0.5 MB g/100 g or a low-fat control diet for 3⁻4 weeks. Lipid profiles in blood and liver tissue, intestinal permeability and cecal short-chain fatty acids were examined. The results showed... (More)

Butyric acid has been shown to reduce high-fat diet-related metabolic disturbances and to improve intestinal barrier function due to its potent anti-inflammatory capacity. This study investigates whether a butyric acid ester, monobutyrin (MB) affects lipid profiles and gut barrier function in a dose-response manner in rats fed butter- or lard-based high-fat diets. Four-week-old male Wistar rats were fed butter-based diets containing 0, 0.25, 0.75 and 1.5 MB g/100 g (dry weight basis) or 0.5 glycerol g/100 g, and diets with lard (La) containing 0 and 0.5 MB g/100 g or a low-fat control diet for 3⁻4 weeks. Lipid profiles in blood and liver tissue, intestinal permeability and cecal short-chain fatty acids were examined. The results showed a dose-dependent decrease in liver total cholesterol for 1.5 MB (p < 0.05) and liver triglycerides for 0.75 MB (p < 0.05) and 1.5 MB (p = 0.08) groups compared to the high-fat control group. Furthermore, a lower excretion of mannitol in urine in the 1.5 MB group indicated improved intestinal barrier function. When MB was supplemented in the lard-based diet, serum total cholesterol levels decreased, and total amount of liver high-density lipoprotein-cholesterol increased. Thus, MB dietary supplementation can be effective in counteracting lipid metabolism disturbances and impaired gut barrier function induced by high-fat diets.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
butter, butyrate, cecal SCFA, intestinal permeability, lactulose/mannitol test, lard, lipid metabolism, Wistar rat
in
Nutrients
volume
11
issue
2
publisher
MDPI AG
external identifiers
  • scopus:85061141973
ISSN
2072-6643
DOI
10.3390/nu11020308
language
English
LU publication?
yes
id
ad707022-5b1a-41d5-b767-cca4f085d0a4
date added to LUP
2019-02-15 08:09:20
date last changed
2019-05-18 03:00:20
@article{ad707022-5b1a-41d5-b767-cca4f085d0a4,
  abstract     = {<p>Butyric acid has been shown to reduce high-fat diet-related metabolic disturbances and to improve intestinal barrier function due to its potent anti-inflammatory capacity. This study investigates whether a butyric acid ester, monobutyrin (MB) affects lipid profiles and gut barrier function in a dose-response manner in rats fed butter- or lard-based high-fat diets. Four-week-old male Wistar rats were fed butter-based diets containing 0, 0.25, 0.75 and 1.5 MB g/100 g (dry weight basis) or 0.5 glycerol g/100 g, and diets with lard (La) containing 0 and 0.5 MB g/100 g or a low-fat control diet for 3⁻4 weeks. Lipid profiles in blood and liver tissue, intestinal permeability and cecal short-chain fatty acids were examined. The results showed a dose-dependent decrease in liver total cholesterol for 1.5 MB (p &lt; 0.05) and liver triglycerides for 0.75 MB (p &lt; 0.05) and 1.5 MB (p = 0.08) groups compared to the high-fat control group. Furthermore, a lower excretion of mannitol in urine in the 1.5 MB group indicated improved intestinal barrier function. When MB was supplemented in the lard-based diet, serum total cholesterol levels decreased, and total amount of liver high-density lipoprotein-cholesterol increased. Thus, MB dietary supplementation can be effective in counteracting lipid metabolism disturbances and impaired gut barrier function induced by high-fat diets.</p>},
  author       = {Nguyen, Thao Duy and Prykhodko, Olena and Hållenius, Frida F. and Nyman, Margareta},
  issn         = {2072-6643},
  keyword      = {butter,butyrate,cecal SCFA,intestinal permeability,lactulose/mannitol test,lard,lipid metabolism,Wistar rat},
  language     = {eng},
  month        = {02},
  number       = {2},
  publisher    = {MDPI AG},
  series       = {Nutrients},
  title        = {Monobutyrin Reduces Liver Cholesterol and Improves Intestinal Barrier Function in Rats Fed High-Fat Diets},
  url          = {http://dx.doi.org/10.3390/nu11020308},
  volume       = {11},
  year         = {2019},
}