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Binding and the effect of the red kidney bean lectin, phytohaemagglutinin, in the gastrointestinal tract of suckling rats

Linderoth, Ann LU ; Prykhodko, Olena LU ; Ahrén, Bo LU ; Fåk, Frida LU ; Pierzynowski, Stefan LU and Weström, Björn LU (2006) In British Journal of Nutrition 95(1). p.105-115
Abstract
Enteral exposure of suckling rats to phytohaemagglutinin (PHA) has been shown to induce growth and precocious functional maturation of the gastrointestinal tract. The aim of the present study was to explore the mechanism of this action. Suckling rats, 14 d old, were fed a single dose of PHA (0.05 mg/g body weight) or saline. The binding of PHA to the gut epithelium and its effect on the morphology and functional properties of the gut and pancreas were studied up to 3 d after treatment. Initially, at 1-24 h, the PHA bound along the gut mucosal lining, resulting in disturbed gut morphology with villi shortening and rapid decreases in disaccharidase activities and macromolecular absorption capacity. During a later phase, between 1 and 3 d,... (More)
Enteral exposure of suckling rats to phytohaemagglutinin (PHA) has been shown to induce growth and precocious functional maturation of the gastrointestinal tract. The aim of the present study was to explore the mechanism of this action. Suckling rats, 14 d old, were fed a single dose of PHA (0.05 mg/g body weight) or saline. The binding of PHA to the gut epithelium and its effect on the morphology and functional properties of the gut and pancreas were studied up to 3 d after treatment. Initially, at 1-24 h, the PHA bound along the gut mucosal lining, resulting in disturbed gut morphology with villi shortening and rapid decreases in disaccharidase activities and macromolecular absorption capacity. During a later phase, between 1 and 3 d, the PHA binding had declined, and an uptake by enterocytes was observed. An increase in crypt cell proliferation and gut growth became evident during this period, together with a functional maturation, as indicated by increases in disaccharidase (maltase and sucrase) activities and the low macromolecular absorption capacity. Pancreas growth also increased, as did its content of digestive enzymes. We conclude that enteral exposure to PHA in suckling rats temporarily causes mucosal disarrangement and functional impediment of the gut, which may be explained by binding to and disruption of the gut mucosa and a two-fold increase in the plasma corticosterone concentration. These findings may lead to a better understanding of the role of diet in gastrointestinal maturation and may constitute a basis for the treatment of mammals having an immature gut. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
pancreas, gut permeability, disaccharidases, endocytosis
in
British Journal of Nutrition
volume
95
issue
1
pages
105 - 115
publisher
Cambridge University Press
external identifiers
  • pmid:16441922
  • wos:000234936600013
  • scopus:32944454375
ISSN
1475-2662
DOI
10.1079/BJN20051612
language
English
LU publication?
yes
id
c38b3991-8575-48b8-998e-7b5bda26792c (old id 419516)
date added to LUP
2007-10-08 15:47:55
date last changed
2019-06-25 01:45:17
@article{c38b3991-8575-48b8-998e-7b5bda26792c,
  abstract     = {Enteral exposure of suckling rats to phytohaemagglutinin (PHA) has been shown to induce growth and precocious functional maturation of the gastrointestinal tract. The aim of the present study was to explore the mechanism of this action. Suckling rats, 14 d old, were fed a single dose of PHA (0.05 mg/g body weight) or saline. The binding of PHA to the gut epithelium and its effect on the morphology and functional properties of the gut and pancreas were studied up to 3 d after treatment. Initially, at 1-24 h, the PHA bound along the gut mucosal lining, resulting in disturbed gut morphology with villi shortening and rapid decreases in disaccharidase activities and macromolecular absorption capacity. During a later phase, between 1 and 3 d, the PHA binding had declined, and an uptake by enterocytes was observed. An increase in crypt cell proliferation and gut growth became evident during this period, together with a functional maturation, as indicated by increases in disaccharidase (maltase and sucrase) activities and the low macromolecular absorption capacity. Pancreas growth also increased, as did its content of digestive enzymes. We conclude that enteral exposure to PHA in suckling rats temporarily causes mucosal disarrangement and functional impediment of the gut, which may be explained by binding to and disruption of the gut mucosa and a two-fold increase in the plasma corticosterone concentration. These findings may lead to a better understanding of the role of diet in gastrointestinal maturation and may constitute a basis for the treatment of mammals having an immature gut.},
  author       = {Linderoth, Ann and Prykhodko, Olena and Ahrén, Bo and Fåk, Frida and Pierzynowski, Stefan and Weström, Björn},
  issn         = {1475-2662},
  keyword      = {pancreas,gut permeability,disaccharidases,endocytosis},
  language     = {eng},
  number       = {1},
  pages        = {105--115},
  publisher    = {Cambridge University Press},
  series       = {British Journal of Nutrition},
  title        = {Binding and the effect of the red kidney bean lectin, phytohaemagglutinin, in the gastrointestinal tract of suckling rats},
  url          = {http://dx.doi.org/10.1079/BJN20051612},
  volume       = {95},
  year         = {2006},
}