Binding and the effect of the red kidney bean lectin, phytohaemagglutinin, in the gastrointestinal tract of suckling rats
Linderoth, Ann LU ; Prykhodko, Olena LU ; Ahrén, Bo LU ; Fåk, Frida LU
; Pierzynowski, Stefan
LU
and Weström, Björn
LU
(2006)
In British Journal of Nutrition
95(1).
p.105-115
- Abstract
- Enteral exposure of suckling rats to phytohaemagglutinin (PHA) has been shown to induce growth and precocious functional maturation of the gastrointestinal tract. The aim of the present study was to explore the mechanism of this action. Suckling rats, 14 d old, were fed a single dose of PHA (0.05 mg/g body weight) or saline. The binding of PHA to the gut epithelium and its effect on the morphology and functional properties of the gut and pancreas were studied up to 3 d after treatment. Initially, at 1-24 h, the PHA bound along the gut mucosal lining, resulting in disturbed gut morphology with villi shortening and rapid decreases in disaccharidase activities and macromolecular absorption capacity. During a later phase, between 1 and 3 d,... (More)
- Enteral exposure of suckling rats to phytohaemagglutinin (PHA) has been shown to induce growth and precocious functional maturation of the gastrointestinal tract. The aim of the present study was to explore the mechanism of this action. Suckling rats, 14 d old, were fed a single dose of PHA (0.05 mg/g body weight) or saline. The binding of PHA to the gut epithelium and its effect on the morphology and functional properties of the gut and pancreas were studied up to 3 d after treatment. Initially, at 1-24 h, the PHA bound along the gut mucosal lining, resulting in disturbed gut morphology with villi shortening and rapid decreases in disaccharidase activities and macromolecular absorption capacity. During a later phase, between 1 and 3 d, the PHA binding had declined, and an uptake by enterocytes was observed. An increase in crypt cell proliferation and gut growth became evident during this period, together with a functional maturation, as indicated by increases in disaccharidase (maltase and sucrase) activities and the low macromolecular absorption capacity. Pancreas growth also increased, as did its content of digestive enzymes. We conclude that enteral exposure to PHA in suckling rats temporarily causes mucosal disarrangement and functional impediment of the gut, which may be explained by binding to and disruption of the gut mucosa and a two-fold increase in the plasma corticosterone concentration. These findings may lead to a better understanding of the role of diet in gastrointestinal maturation and may constitute a basis for the treatment of mammals having an immature gut. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/419516
- author
- Linderoth, Ann
LU
; Prykhodko, Olena
LU
; Ahrén, Bo
LU
; Fåk, Frida
LU
; Pierzynowski, Stefan
LU
and Weström, Björn
LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- pancreas, gut permeability, disaccharidases, endocytosis
- in
- British Journal of Nutrition
- volume
- 95
- issue
- 1
- pages
- 105 - 115
- publisher
- Cambridge University Press
- external identifiers
-
- pmid:16441922
- wos:000234936600013
- scopus:32944454375
- ISSN
- 1475-2662
- DOI
- 10.1079/BJN20051612
- language
- English
- LU publication?
- yes
- id
- c38b3991-8575-48b8-998e-7b5bda26792c (old id 419516)
- date added to LUP
- 2016-04-01 12:38:12
- date last changed
- 2024-03-12 18:57:44
@article{c38b3991-8575-48b8-998e-7b5bda26792c,
abstract = {{Enteral exposure of suckling rats to phytohaemagglutinin (PHA) has been shown to induce growth and precocious functional maturation of the gastrointestinal tract. The aim of the present study was to explore the mechanism of this action. Suckling rats, 14 d old, were fed a single dose of PHA (0.05 mg/g body weight) or saline. The binding of PHA to the gut epithelium and its effect on the morphology and functional properties of the gut and pancreas were studied up to 3 d after treatment. Initially, at 1-24 h, the PHA bound along the gut mucosal lining, resulting in disturbed gut morphology with villi shortening and rapid decreases in disaccharidase activities and macromolecular absorption capacity. During a later phase, between 1 and 3 d, the PHA binding had declined, and an uptake by enterocytes was observed. An increase in crypt cell proliferation and gut growth became evident during this period, together with a functional maturation, as indicated by increases in disaccharidase (maltase and sucrase) activities and the low macromolecular absorption capacity. Pancreas growth also increased, as did its content of digestive enzymes. We conclude that enteral exposure to PHA in suckling rats temporarily causes mucosal disarrangement and functional impediment of the gut, which may be explained by binding to and disruption of the gut mucosa and a two-fold increase in the plasma corticosterone concentration. These findings may lead to a better understanding of the role of diet in gastrointestinal maturation and may constitute a basis for the treatment of mammals having an immature gut.}},
author = {{Linderoth, Ann and Prykhodko, Olena and Ahrén, Bo and Fåk, Frida and Pierzynowski, Stefan and Weström, Björn}},
issn = {{1475-2662}},
keywords = {{pancreas; gut permeability; disaccharidases; endocytosis}},
language = {{eng}},
number = {{1}},
pages = {{105--115}},
publisher = {{Cambridge University Press}},
series = {{British Journal of Nutrition}},
title = {{Binding and the effect of the red kidney bean lectin, phytohaemagglutinin, in the gastrointestinal tract of suckling rats}},
url = {{http://dx.doi.org/10.1079/BJN20051612}},
doi = {{10.1079/BJN20051612}},
volume = {{95}},
year = {{2006}},
}