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Identification of Phlorotannins in the Brown Algae, Saccharina latissima and Ascophyllum nodosum by Ultra-High-Performance Liquid Chromatography Coupled to High-Resolution Tandem Mass Spectrometry

Sardari, Roya R. R. LU ; Prothmann, Jens LU ; Gregersen, Olavur ; Turner, Charlotta LU orcid and Nordberg Karlsson, Eva LU orcid (2021) In Molecules (Basel, Switzerland) 26(1).
Abstract

Phlorotannins are bioactive polyphenols in brown macroalgae that make these algae interesting as healthy food. Specific phlorotannins are, however, seldom identified, and extracts from different species are often only analysed for total phenolic content (TPC). In this study, our focus was to identify phlorotannin molecules from Saccharina latissima and Ascophyllum nodosum (a species rich in these compounds) using ultra-high-performance liquid chromatography coupled to high-resolution tandem mass spectrometry (UHPLC-HRMS2). Water and ethanol (30 and 80% v/v) were used at solid:liquid ratios, extraction times and temperatures, proposed to result in high TPC in extracts from other species. The S.... (More)

Phlorotannins are bioactive polyphenols in brown macroalgae that make these algae interesting as healthy food. Specific phlorotannins are, however, seldom identified, and extracts from different species are often only analysed for total phenolic content (TPC). In this study, our focus was to identify phlorotannin molecules from Saccharina latissima and Ascophyllum nodosum (a species rich in these compounds) using ultra-high-performance liquid chromatography coupled to high-resolution tandem mass spectrometry (UHPLC-HRMS2). Water and ethanol (30 and 80% v/v) were used at solid:liquid ratios, extraction times and temperatures, proposed to result in high TPC in extracts from other species. The S. latissima extracts, however, did not allow phlorotannin detection by either UHPLC-UV/Vis or UHPLC-HRMS2, despite a TPC response by the Folin-Ciocalteu assay, pinpointing a problem with interference by non-phenolic compounds. Purification by solid phase extraction (SPE) led to purer, more concentrated fractions and identification of four phlorotannin species in A. nodosum and one in S. latissima by UHPLC-HRMS2, using extracts in ethanol 80% v/v at a solid:liquid ratio of 1:10 for 20 h at 25 °C with an added 10 h at 65 °C incubation of remaining solids. The phlorotannin with the formula C12H10O7 (corresponding to bifuhalol) is the first identified in S. latissima.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Extraction, Saccharina latissima, Ascophyllum nodosum, Brown algae, Polyphenols, Phlorotannins, High-resolution mass spectrometry
in
Molecules (Basel, Switzerland)
volume
26
issue
1
article number
43
pages
17 pages
publisher
MDPI AG
external identifiers
  • pmid:33374856
  • scopus:85099114742
ISSN
1420-3049
DOI
10.3390/molecules26010043
language
English
LU publication?
yes
id
c6c28607-dd60-43a2-80b7-58b5c074455d
date added to LUP
2021-01-07 12:24:51
date last changed
2024-06-27 05:05:50
@article{c6c28607-dd60-43a2-80b7-58b5c074455d,
  abstract     = {{<p>Phlorotannins are bioactive polyphenols in brown macroalgae that make these algae interesting as healthy food. Specific phlorotannins are, however, seldom identified, and extracts from different species are often only analysed for total phenolic content (TPC). In this study, our focus was to identify phlorotannin molecules from <i>Saccharina latissima</i> and <i>Ascophyllum nodosum</i> (a species rich in these compounds) using ultra-high-performance liquid chromatography coupled to high-resolution tandem mass spectrometry (UHPLC-HRMS<sup>2</sup>). Water and ethanol (30 and 80% <i>v</i>/<i>v</i>) were used at solid:liquid ratios, extraction times and temperatures, proposed to result in high TPC in extracts from other species. The <i>S. latissima extracts</i>, however, did not allow phlorotannin detection by either UHPLC-UV/Vis or UHPLC-HRMS<sup>2</sup>, despite a TPC response by the Folin-Ciocalteu assay, pinpointing a problem with interference by non-phenolic compounds. Purification by solid phase extraction (SPE) led to purer, more concentrated fractions and identification of four phlorotannin species in <i>A. nodosum</i> and one in <i>S. latissima</i> by UHPLC-HRMS<sup>2</sup>, using extracts in ethanol 80% <i>v</i>/<i>v</i> at a solid:liquid ratio of 1:10 for 20 h at 25 °C with an added 10 h at 65 °C incubation of remaining solids. The phlorotannin with the formula C12H10O7 (corresponding to bifuhalol) is the first identified in <i>S. latissima</i>.</p>}},
  author       = {{Sardari, Roya R. R. and Prothmann, Jens and Gregersen, Olavur and Turner, Charlotta and Nordberg Karlsson, Eva}},
  issn         = {{1420-3049}},
  keywords     = {{Extraction; Saccharina latissima; Ascophyllum  nodosum; Brown algae; Polyphenols; Phlorotannins; High-resolution mass spectrometry}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{MDPI AG}},
  series       = {{Molecules (Basel, Switzerland)}},
  title        = {{Identification of Phlorotannins in the Brown Algae, Saccharina latissima and Ascophyllum nodosum by Ultra-High-Performance Liquid Chromatography Coupled to High-Resolution Tandem Mass Spectrometry}},
  url          = {{http://dx.doi.org/10.3390/molecules26010043}},
  doi          = {{10.3390/molecules26010043}},
  volume       = {{26}},
  year         = {{2021}},
}