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Heparan/chondroitin/dermatan sulfate primer 2-(6-hydroxynaphthyl)-O-beta-D-xylopyranoside preferentially inhibits growth of transformed cells

Mani, K LU ; Havsmark, B LU ; Persson, Susanne; Kaneda, Y; Yamamoto, H; Sakurai, K; Ashikari, S; Habuchi, H; Suzuki, S and Kimata, K, et al. (1998) In Cancer Research 58(6). p.104-1099
Abstract

Xylose forms the direct carbohydrate-protein link in extra- or pericellular proteoglycans (PGs) that are substituted with either chondroitin sulfate (CS)/dermatan sulfate (DS) and/or heparan sulfate (HS). Cell surface PGs carrying HS are important regulators of cell growth. Xylose coupled to an aromatic compound can enter cells and initiate either CS/DS synthesis or both HS and CS/DS synthesis, depending on the nature of the aromatic adduct. Here, we show that 2-(6-hydroxynaphthyl)-O-beta-D-xylopyranoside, which can prime both types of glycan chains, inhibits growth of a set of normal and transformed cells. Transformed cells are preferentially inhibited, and at a concentration of 0.15-0.20 mM xyloside, transformed cells are totally... (More)

Xylose forms the direct carbohydrate-protein link in extra- or pericellular proteoglycans (PGs) that are substituted with either chondroitin sulfate (CS)/dermatan sulfate (DS) and/or heparan sulfate (HS). Cell surface PGs carrying HS are important regulators of cell growth. Xylose coupled to an aromatic compound can enter cells and initiate either CS/DS synthesis or both HS and CS/DS synthesis, depending on the nature of the aromatic adduct. Here, we show that 2-(6-hydroxynaphthyl)-O-beta-D-xylopyranoside, which can prime both types of glycan chains, inhibits growth of a set of normal and transformed cells. Transformed cells are preferentially inhibited, and at a concentration of 0.15-0.20 mM xyloside, transformed cells are totally growth arrested, whereas normal cells are only < or = 50% inhibited. No inhibition of growth is observed with the stereoisomeric 2-(6-hydroxynaphthyl)-O-beta-L-xylopyranoside, which does not prime glycosaminoglycan synthesis at all; with the nonhydroxylated 2-naphthyl-O-beta-D-xylopyranoside, which only primes CS/DS synthesis under these conditions; or with p-nitrophenyl-O-beta-D-xylopyranoside, which is known to prime only CS/DS synthesis. We conclude that growth inhibition is due to priming of HS and/or CS/DS synthesis, which may either lead to the formation of specific antiproliferative glycans or glycan fragments or to interference with endogenous PG synthesis and turnover.

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3T3 Cells, Animals, Chondroitin Sulfates, Dermatan Sulfate, Endothelium, Vascular, Glycosides, Growth Inhibitors, Humans, Mice, Naphthols, Stereoisomerism, Tumor Cells, Cultured, Journal Article, Research Support, Non-U.S. Gov't
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Cancer Research
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58
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6
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6 pages
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American Association for Cancer Research Inc.
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  • scopus:0032521158
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0008-5472
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English
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yes
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d87f239a-f6b2-4566-9d01-fa6f333b23ba
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2017-06-27 14:13:47
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2017-07-02 05:03:53
@article{d87f239a-f6b2-4566-9d01-fa6f333b23ba,
  abstract     = {<p>Xylose forms the direct carbohydrate-protein link in extra- or pericellular proteoglycans (PGs) that are substituted with either chondroitin sulfate (CS)/dermatan sulfate (DS) and/or heparan sulfate (HS). Cell surface PGs carrying HS are important regulators of cell growth. Xylose coupled to an aromatic compound can enter cells and initiate either CS/DS synthesis or both HS and CS/DS synthesis, depending on the nature of the aromatic adduct. Here, we show that 2-(6-hydroxynaphthyl)-O-beta-D-xylopyranoside, which can prime both types of glycan chains, inhibits growth of a set of normal and transformed cells. Transformed cells are preferentially inhibited, and at a concentration of 0.15-0.20 mM xyloside, transformed cells are totally growth arrested, whereas normal cells are only &lt; or = 50% inhibited. No inhibition of growth is observed with the stereoisomeric 2-(6-hydroxynaphthyl)-O-beta-L-xylopyranoside, which does not prime glycosaminoglycan synthesis at all; with the nonhydroxylated 2-naphthyl-O-beta-D-xylopyranoside, which only primes CS/DS synthesis under these conditions; or with p-nitrophenyl-O-beta-D-xylopyranoside, which is known to prime only CS/DS synthesis. We conclude that growth inhibition is due to priming of HS and/or CS/DS synthesis, which may either lead to the formation of specific antiproliferative glycans or glycan fragments or to interference with endogenous PG synthesis and turnover.</p>},
  author       = {Mani, K and Havsmark, B and Persson, Susanne and Kaneda, Y and Yamamoto, H and Sakurai, K and Ashikari, S and Habuchi, H and Suzuki, S and Kimata, K and Malmström, A and Westergren-Thorsson, G and Fransson, L A},
  issn         = {0008-5472},
  keyword      = {3T3 Cells,Animals,Chondroitin Sulfates,Dermatan Sulfate,Endothelium, Vascular,Glycosides,Growth Inhibitors,Humans,Mice,Naphthols,Stereoisomerism,Tumor Cells, Cultured,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {03},
  number       = {6},
  pages        = {104--1099},
  publisher    = {American Association for Cancer Research Inc.},
  series       = {Cancer Research},
  title        = {Heparan/chondroitin/dermatan sulfate primer 2-(6-hydroxynaphthyl)-O-beta-D-xylopyranoside preferentially inhibits growth of transformed cells},
  volume       = {58},
  year         = {1998},
}