Dipeptidyl peptidase 4 expression is not associated with an activated fibroblast phenotype in idiopathic pulmonary fibrosis
(2022) In Frontiers in Pharmacology 13. p.1-12- Abstract
Dipeptidyl peptidase 4 (DPP4) has been proposed as a marker for activated fibroblasts in fibrotic disease. We aimed to investigate whether a profibrotic DPP4 phenotype is present in lung tissue from patients with idiopathic pulmonary fibrosis (IPF). The presence of DPP4
+ fibroblasts in normal and IPF lung tissue was investigated using flow cytometry and immunohistology. In addition, the involvement of DPP4 in fibroblast activation was examined
in vitro, using CRISPR/Cas9 mediated genetic inactivation to generate primary DPP4 knockout lung fibroblasts. We observed a reduced frequency of primary DPP4
+ fibroblasts in IPF tissue using flow cytometry, and an absence of DPP4
+ fibroblasts in pathohistological features... (More)Dipeptidyl peptidase 4 (DPP4) has been proposed as a marker for activated fibroblasts in fibrotic disease. We aimed to investigate whether a profibrotic DPP4 phenotype is present in lung tissue from patients with idiopathic pulmonary fibrosis (IPF). The presence of DPP4
(Less)
+ fibroblasts in normal and IPF lung tissue was investigated using flow cytometry and immunohistology. In addition, the involvement of DPP4 in fibroblast activation was examined
in vitro, using CRISPR/Cas9 mediated genetic inactivation to generate primary DPP4 knockout lung fibroblasts. We observed a reduced frequency of primary DPP4
+ fibroblasts in IPF tissue using flow cytometry, and an absence of DPP4
+ fibroblasts in pathohistological features of IPF. The
in vivo observations were supported by results
in vitro showing a decreased expression of DPP4 on normal and IPF fibroblasts after profibrotic stimuli (transforming growth factor β) and no effect on the expression of activation markers (α-smooth muscle actin, collagen I and connective tissue growth factor) upon knockout of DPP4 in lung fibroblasts with or without activation with profibrotic stimuli.
- author
- Kadefors, Måns LU ; Berlin, Frida LU ; Wildt, Marie LU ; Dellgren, Göran ; Rolandsson Enes, Sara LU ; Aspberg, Anders LU and Westergren-Thorsson, Gunilla LU
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Frontiers in Pharmacology
- volume
- 13
- article number
- 953771
- pages
- 1 - 12
- publisher
- Frontiers Media S. A.
- external identifiers
-
- scopus:85137973924
- pmid:36120350
- ISSN
- 1663-9812
- DOI
- 10.3389/fphar.2022.953771
- project
- Characterization of lung-derived mesenchymal stromal cells
- language
- English
- LU publication?
- yes
- additional info
- Copyright © 2022 Kadefors, Berlin, Wildt, Dellgren, Rolandsson Enes, Aspberg and Westergren-Thorsson.
- id
- d9371285-3ac6-44c8-a078-40b4c9565973
- date added to LUP
- 2022-09-22 17:20:39
- date last changed
- 2024-09-18 05:00:03
@article{d9371285-3ac6-44c8-a078-40b4c9565973, abstract = {{<p>Dipeptidyl peptidase 4 (DPP4) has been proposed as a marker for activated fibroblasts in fibrotic disease. We aimed to investigate whether a profibrotic DPP4 phenotype is present in lung tissue from patients with idiopathic pulmonary fibrosis (IPF). The presence of DPP4<br> + fibroblasts in normal and IPF lung tissue was investigated using flow cytometry and immunohistology. In addition, the involvement of DPP4 in fibroblast activation was examined <br> in vitro, using CRISPR/Cas9 mediated genetic inactivation to generate primary DPP4 knockout lung fibroblasts. We observed a reduced frequency of primary DPP4<br> + fibroblasts in IPF tissue using flow cytometry, and an absence of DPP4<br> + fibroblasts in pathohistological features of IPF. The <br> in vivo observations were supported by results <br> in vitro showing a decreased expression of DPP4 on normal and IPF fibroblasts after profibrotic stimuli (transforming growth factor β) and no effect on the expression of activation markers (α-smooth muscle actin, collagen I and connective tissue growth factor) upon knockout of DPP4 in lung fibroblasts with or without activation with profibrotic stimuli.<br> </p>}}, author = {{Kadefors, Måns and Berlin, Frida and Wildt, Marie and Dellgren, Göran and Rolandsson Enes, Sara and Aspberg, Anders and Westergren-Thorsson, Gunilla}}, issn = {{1663-9812}}, language = {{eng}}, pages = {{1--12}}, publisher = {{Frontiers Media S. A.}}, series = {{Frontiers in Pharmacology}}, title = {{Dipeptidyl peptidase 4 expression is not associated with an activated fibroblast phenotype in idiopathic pulmonary fibrosis}}, url = {{http://dx.doi.org/10.3389/fphar.2022.953771}}, doi = {{10.3389/fphar.2022.953771}}, volume = {{13}}, year = {{2022}}, }