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Immunological alteration and changes of gut microbiota after dextran sulfate sodium (DSS) administration in mice

Håkansson, Åsa LU ; Tormo-Badia, Neivis LU ; Baridi, Ajoeb LU ; Xu, Jie LU ; Molin, Göran LU ; Johansson Hagslätt, Marie-Louise; Linninge, Caroline LU ; JEPPSSON, BENGT LU ; Cilio, Corrado LU and Ahrné, Siv LU (2014) In Clinical and Experimental Medicine 15(1). p.107-120
Abstract
Ulcerative colitis (UC) is characterized by
chronic inflammation of the colonic mucosa. Administration
of dextran sulfate sodium (DSS) to animals is a frequently
used model to mimic human colitis. Deregulation
of the immune response to the enteric microflora or
pathogens as well as increased intestinal permeability have
been proposed as disease-driving mechanisms. To enlarge
the understanding of the pathogenesis, we have studied the
effect of DSS on the immune system and gut microbiota in
mice. Intestinal inflammation was verified through histological
evaluation and myeloperoxidase activity. Immunological
changes were assessed by flow cytometry in
spleen, Peyer0s patches and mesenteric lymph... (More)
Ulcerative colitis (UC) is characterized by
chronic inflammation of the colonic mucosa. Administration
of dextran sulfate sodium (DSS) to animals is a frequently
used model to mimic human colitis. Deregulation
of the immune response to the enteric microflora or
pathogens as well as increased intestinal permeability have
been proposed as disease-driving mechanisms. To enlarge
the understanding of the pathogenesis, we have studied the
effect of DSS on the immune system and gut microbiota in
mice. Intestinal inflammation was verified through histological
evaluation and myeloperoxidase activity. Immunological
changes were assessed by flow cytometry in
spleen, Peyer0s patches and mesenteric lymph nodes and
through multiplex cytokine profiling. In addition, quantification
of the total amount of bacteria on colonic mucosa
as well as the total amount of lactobacilli, Akkermansia,
Desulfovibrio and Enterobacteriaceae was performed by
the use of quantitative PCR. Diversity and community
structure were analysed by terminal restriction fragment
length polymorphism (T-RFLP) patterns, and principal
component analysis was utilized on immunological and
T-RFLP patterns. DSS-induced colitis show clinical and
histological similarities to UC. The composition of the
colonic microflora was profoundly changed and correlated
with several alterations of the immune system. The results
demonstrate a relationship between multiple immunological
changes and alterations of the gut microbiota after DSS
administration. These data highlight and improve the definition
of the immunological basis of the disease and
suggest a role for dysregulation of the gut microbiota in the
pathogenesis of colitis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical and Experimental Medicine
volume
15
issue
1
pages
107 - 120
publisher
Springer
external identifiers
  • scopus:84902262539
ISSN
1591-9528
DOI
10.1007/s10238-013-0270-5
language
English
LU publication?
yes
id
db676fa1-d446-4819-b020-3af7199664c1
date added to LUP
2016-10-03 21:33:15
date last changed
2017-10-01 05:24:04
@article{db676fa1-d446-4819-b020-3af7199664c1,
  abstract     = {Ulcerative colitis (UC) is characterized by<br/>chronic inflammation of the colonic mucosa. Administration<br/>of dextran sulfate sodium (DSS) to animals is a frequently<br/>used model to mimic human colitis. Deregulation<br/>of the immune response to the enteric microflora or<br/>pathogens as well as increased intestinal permeability have<br/>been proposed as disease-driving mechanisms. To enlarge<br/>the understanding of the pathogenesis, we have studied the<br/>effect of DSS on the immune system and gut microbiota in<br/>mice. Intestinal inflammation was verified through histological<br/>evaluation and myeloperoxidase activity. Immunological<br/>changes were assessed by flow cytometry in<br/>spleen, Peyer0s patches and mesenteric lymph nodes and<br/>through multiplex cytokine profiling. In addition, quantification<br/>of the total amount of bacteria on colonic mucosa<br/>as well as the total amount of lactobacilli, Akkermansia,<br/>Desulfovibrio and Enterobacteriaceae was performed by<br/>the use of quantitative PCR. Diversity and community<br/>structure were analysed by terminal restriction fragment<br/>length polymorphism (T-RFLP) patterns, and principal<br/>component analysis was utilized on immunological and<br/>T-RFLP patterns. DSS-induced colitis show clinical and<br/>histological similarities to UC. The composition of the<br/>colonic microflora was profoundly changed and correlated<br/>with several alterations of the immune system. The results<br/>demonstrate a relationship between multiple immunological<br/>changes and alterations of the gut microbiota after DSS<br/>administration. These data highlight and improve the definition<br/>of the immunological basis of the disease and<br/>suggest a role for dysregulation of the gut microbiota in the<br/>pathogenesis of colitis.},
  author       = {Håkansson, Åsa and Tormo-Badia, Neivis and Baridi, Ajoeb and Xu, Jie and Molin, Göran and Johansson Hagslätt, Marie-Louise and Linninge, Caroline and JEPPSSON, BENGT and Cilio, Corrado and Ahrné, Siv},
  issn         = {1591-9528},
  language     = {eng},
  number       = {1},
  pages        = {107--120},
  publisher    = {Springer},
  series       = {Clinical and Experimental Medicine},
  title        = {Immunological alteration and changes of gut microbiota after dextran sulfate sodium (DSS) administration in mice},
  url          = {http://dx.doi.org/10.1007/s10238-013-0270-5},
  volume       = {15},
  year         = {2014},
}