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Induction of Vesicle Formation by Exposing Apple Tissue to Vacuum Impregnation

Tylewicz, Urszula; Romani, Santina; Widell, Susanne LU and Gomez, Federico LU (2013) In Food and Bioprocess Technology 6(4). p.1099-1104
Abstract
This paper demonstrates that vacuum impregnation of mature apple tissue in the presence of different sugars results in the formation of membrane vesicles inside the cells. Vesiculation is regarded to be a metabolic consequence of the impregnation process. Vesiculation is shown when the endocytic marker FM4-64 was impregnated into the apple tissue together with the sugar solutions. Vesicles were formed at the plasma membrane already 30 min after impregnation and remained inside the cells for at least 24 h, a metabolic process that was inhibited in the presence of chloroquine, a specific endosomic inhibitor. This phenomenon was not dependent on the osmotic strength when sucrose was used for impregnation. However, the vesiculation drastically... (More)
This paper demonstrates that vacuum impregnation of mature apple tissue in the presence of different sugars results in the formation of membrane vesicles inside the cells. Vesiculation is regarded to be a metabolic consequence of the impregnation process. Vesiculation is shown when the endocytic marker FM4-64 was impregnated into the apple tissue together with the sugar solutions. Vesicles were formed at the plasma membrane already 30 min after impregnation and remained inside the cells for at least 24 h, a metabolic process that was inhibited in the presence of chloroquine, a specific endosomic inhibitor. This phenomenon was not dependent on the osmotic strength when sucrose was used for impregnation. However, the vesiculation drastically dropped when a hypertonic trehalose solution was impregnated. We suggest that the impregnated sugars may not totally remain in the extracellular space between the cells, as normally believed, but at least a fraction might be incorporated into the cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Vacuum impregnation, Vesiculation, Endocytosis, Apple
in
Food and Bioprocess Technology
volume
6
issue
4
pages
1099 - 1104
publisher
Springer
external identifiers
  • wos:000315486400023
  • scopus:84874510725
ISSN
1935-5149
DOI
10.1007/s11947-011-0644-1
language
English
LU publication?
yes
id
e6fe27c2-7530-465a-86a0-3787246083ab (old id 3636042)
date added to LUP
2013-04-24 12:40:03
date last changed
2019-03-31 03:13:56
@article{e6fe27c2-7530-465a-86a0-3787246083ab,
  abstract     = {This paper demonstrates that vacuum impregnation of mature apple tissue in the presence of different sugars results in the formation of membrane vesicles inside the cells. Vesiculation is regarded to be a metabolic consequence of the impregnation process. Vesiculation is shown when the endocytic marker FM4-64 was impregnated into the apple tissue together with the sugar solutions. Vesicles were formed at the plasma membrane already 30 min after impregnation and remained inside the cells for at least 24 h, a metabolic process that was inhibited in the presence of chloroquine, a specific endosomic inhibitor. This phenomenon was not dependent on the osmotic strength when sucrose was used for impregnation. However, the vesiculation drastically dropped when a hypertonic trehalose solution was impregnated. We suggest that the impregnated sugars may not totally remain in the extracellular space between the cells, as normally believed, but at least a fraction might be incorporated into the cells.},
  author       = {Tylewicz, Urszula and Romani, Santina and Widell, Susanne and Gomez, Federico},
  issn         = {1935-5149},
  keyword      = {Vacuum impregnation,Vesiculation,Endocytosis,Apple},
  language     = {eng},
  number       = {4},
  pages        = {1099--1104},
  publisher    = {Springer},
  series       = {Food and Bioprocess Technology},
  title        = {Induction of Vesicle Formation by Exposing Apple Tissue to Vacuum Impregnation},
  url          = {http://dx.doi.org/10.1007/s11947-011-0644-1},
  volume       = {6},
  year         = {2013},
}