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Structure dependent antioxidant capacity of phlorotannins from Icelandic Fucus vesiculosus by UHPLC-DAD-ECD-QTOFMS

Hermund, Ditte B.; Plaza, Merichel LU ; Turner, Charlotta LU ; Jónsdóttir, Rosa; Kristinsson, Hordur G.; Jacobsen, Charlotte and Nielsen, Kristian Fog (2018) In Food Chemistry 240. p.904-909
Abstract

Brown algae are rich in polyphenolic compounds, phlorotannins, which have been found to possess high in vitro antioxidant capacity, especially DPPH radical scavenging activity, due to the high number of hydroxyl groups. Whereas, the overall antioxidant capacity of brown algae extracts has been widely studied, the antioxidant capacity of individual phlorotannins has been rarely explored. The aim of this study was to determine the structure dependant antioxidant capacity of phlorotannins from Icelandic brown algae, Fucus vesiculosus. The antioxidant capacity of individual phlorotannins was determined by an on-line method using liquid chromatography and an electrochemical detector followed by quadrupole Time of Flight mass spectrometry... (More)

Brown algae are rich in polyphenolic compounds, phlorotannins, which have been found to possess high in vitro antioxidant capacity, especially DPPH radical scavenging activity, due to the high number of hydroxyl groups. Whereas, the overall antioxidant capacity of brown algae extracts has been widely studied, the antioxidant capacity of individual phlorotannins has been rarely explored. The aim of this study was to determine the structure dependant antioxidant capacity of phlorotannins from Icelandic brown algae, Fucus vesiculosus. The antioxidant capacity of individual phlorotannins was determined by an on-line method using liquid chromatography and an electrochemical detector followed by quadrupole Time of Flight mass spectrometry (UHPLC-DAD-ECD-QTOFMS). Tentative structural elucidation of 13 phlorotannin isomers from EAF was obtained by LC-DAD-QTOFMS, ranging from 374 to 870 Da. On-line determination of antioxidant capacity of the individual phlorotannins generally showed that low molecular phlorotannins exhibited higher antioxidant capacity and that the capacity decreased with polymerisation.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Brown algae, Free radical scavenging, MS/MS, Screening
in
Food Chemistry
volume
240
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:85028310838
ISSN
0308-8146
DOI
10.1016/j.foodchem.2017.08.032
language
English
LU publication?
yes
id
f9014218-31b5-4946-ad5e-5ed1c24650d9
date added to LUP
2017-10-06 11:50:49
date last changed
2018-01-07 12:21:03
@article{f9014218-31b5-4946-ad5e-5ed1c24650d9,
  abstract     = {<p>Brown algae are rich in polyphenolic compounds, phlorotannins, which have been found to possess high in vitro antioxidant capacity, especially DPPH radical scavenging activity, due to the high number of hydroxyl groups. Whereas, the overall antioxidant capacity of brown algae extracts has been widely studied, the antioxidant capacity of individual phlorotannins has been rarely explored. The aim of this study was to determine the structure dependant antioxidant capacity of phlorotannins from Icelandic brown algae, Fucus vesiculosus. The antioxidant capacity of individual phlorotannins was determined by an on-line method using liquid chromatography and an electrochemical detector followed by quadrupole Time of Flight mass spectrometry (UHPLC-DAD-ECD-QTOFMS). Tentative structural elucidation of 13 phlorotannin isomers from EAF was obtained by LC-DAD-QTOFMS, ranging from 374 to 870 Da. On-line determination of antioxidant capacity of the individual phlorotannins generally showed that low molecular phlorotannins exhibited higher antioxidant capacity and that the capacity decreased with polymerisation.</p>},
  author       = {Hermund, Ditte B. and Plaza, Merichel and Turner, Charlotta and Jónsdóttir, Rosa and Kristinsson, Hordur G. and Jacobsen, Charlotte and Nielsen, Kristian Fog},
  issn         = {0308-8146},
  keyword      = {Brown algae,Free radical scavenging,MS/MS,Screening},
  language     = {eng},
  month        = {02},
  pages        = {904--909},
  publisher    = {Elsevier},
  series       = {Food Chemistry},
  title        = {Structure dependent antioxidant capacity of phlorotannins from Icelandic Fucus vesiculosus by UHPLC-DAD-ECD-QTOFMS},
  url          = {http://dx.doi.org/10.1016/j.foodchem.2017.08.032},
  volume       = {240},
  year         = {2018},
}