Reversible Electroporation and Post-Electroporation Resting of Thai Basil Leaves Prior to Convective and Vacuum Drying
(2022) In Applied Sciences (Switzerland) 12(5).- Abstract
Pretreatment by reversible electroporation followed by resting (storage under saturated moisture at 21 ± 2 °C) was evaluated for modification of the properties of dried and rehydrated Thai basil leaves. The treated leaves were dried by convection at 40 °C or in a vacuum at room temperature. The results showed that vacuum drying provoked more cell damage and tissue collapse than convective air drying at a moisture ratio (MR) of 0.2 and 0.1. Under this level of MR, the pulsed electric field (PEF) and resting pretreatment exerts a protective effect of the tissue for both drying methods. However, under complete dehydration (water activity, aw = 0.05) damage seems to be similar for both drying methods despite the PEF pretreatment.... (More)
Pretreatment by reversible electroporation followed by resting (storage under saturated moisture at 21 ± 2 °C) was evaluated for modification of the properties of dried and rehydrated Thai basil leaves. The treated leaves were dried by convection at 40 °C or in a vacuum at room temperature. The results showed that vacuum drying provoked more cell damage and tissue collapse than convective air drying at a moisture ratio (MR) of 0.2 and 0.1. Under this level of MR, the pulsed electric field (PEF) and resting pretreatment exerts a protective effect of the tissue for both drying methods. However, under complete dehydration (water activity, aw = 0.05) damage seems to be similar for both drying methods despite the PEF pretreatment. Remarkably, reversible electroporation followed by resting resulted in higher trichome preservation. At MR of 0.05, the area of trichomes on the surface of convective-dried, PEF-rested and fresh samples were not statistically different at 2267 ± 89 µm2 and 2218 ± 65 µm2, respectively, showing that this pretreatment still exerts a protective effect on trichomes when complete dehydration is achieved.
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- author
- Thamkaew, Grant
LU
; Rasmusson, Allan G.
LU
; Orlov, Dmytro
LU
and Galindo, Federico Gómez LU
- organization
- publishing date
- 2022-03-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Drying methods, Pulsed electric field, Stress response, Thai basil, Trichomes integrity
- in
- Applied Sciences (Switzerland)
- volume
- 12
- issue
- 5
- article number
- 2343
- publisher
- MDPI AG
- external identifiers
-
- scopus:85125306636
- ISSN
- 2076-3417
- DOI
- 10.3390/app12052343
- project
- Advancing optical microscopy for materials engineering in Lund University
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
- id
- 06468c48-bf3b-48d5-9eaa-9a8326ea6529
- date added to LUP
- 2022-03-20 17:59:16
- date last changed
- 2023-12-09 20:03:10
@article{06468c48-bf3b-48d5-9eaa-9a8326ea6529, abstract = {{<p>Pretreatment by reversible electroporation followed by resting (storage under saturated moisture at 21 ± 2 °C) was evaluated for modification of the properties of dried and rehydrated Thai basil leaves. The treated leaves were dried by convection at 40 °C or in a vacuum at room temperature. The results showed that vacuum drying provoked more cell damage and tissue collapse than convective air drying at a moisture ratio (MR) of 0.2 and 0.1. Under this level of MR, the pulsed electric field (PEF) and resting pretreatment exerts a protective effect of the tissue for both drying methods. However, under complete dehydration (water activity, aw = 0.05) damage seems to be similar for both drying methods despite the PEF pretreatment. Remarkably, reversible electroporation followed by resting resulted in higher trichome preservation. At MR of 0.05, the area of trichomes on the surface of convective-dried, PEF-rested and fresh samples were not statistically different at 2267 ± 89 µm<sup>2</sup> and 2218 ± 65 µm<sup>2</sup>, respectively, showing that this pretreatment still exerts a protective effect on trichomes when complete dehydration is achieved.</p>}}, author = {{Thamkaew, Grant and Rasmusson, Allan G. and Orlov, Dmytro and Galindo, Federico Gómez}}, issn = {{2076-3417}}, keywords = {{Drying methods; Pulsed electric field; Stress response; Thai basil; Trichomes integrity}}, language = {{eng}}, month = {{03}}, number = {{5}}, publisher = {{MDPI AG}}, series = {{Applied Sciences (Switzerland)}}, title = {{Reversible Electroporation and Post-Electroporation Resting of Thai Basil Leaves Prior to Convective and Vacuum Drying}}, url = {{http://dx.doi.org/10.3390/app12052343}}, doi = {{10.3390/app12052343}}, volume = {{12}}, year = {{2022}}, }