Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Reversible Electroporation and Post-Electroporation Resting of Thai Basil Leaves Prior to Convective and Vacuum Drying

Thamkaew, Grant LU ; Rasmusson, Allan G. LU ; Orlov, Dmytro LU orcid and Galindo, Federico Gómez LU (2022) In Applied Sciences (Switzerland) 12(5).
Abstract

Pretreatment by reversible electroporation followed by resting (storage under saturated moisture at 21 ± 2 °C) was evaluated for modification of the properties of dried and rehydrated Thai basil leaves. The treated leaves were dried by convection at 40 °C or in a vacuum at room temperature. The results showed that vacuum drying provoked more cell damage and tissue collapse than convective air drying at a moisture ratio (MR) of 0.2 and 0.1. Under this level of MR, the pulsed electric field (PEF) and resting pretreatment exerts a protective effect of the tissue for both drying methods. However, under complete dehydration (water activity, aw = 0.05) damage seems to be similar for both drying methods despite the PEF pretreatment.... (More)

Pretreatment by reversible electroporation followed by resting (storage under saturated moisture at 21 ± 2 °C) was evaluated for modification of the properties of dried and rehydrated Thai basil leaves. The treated leaves were dried by convection at 40 °C or in a vacuum at room temperature. The results showed that vacuum drying provoked more cell damage and tissue collapse than convective air drying at a moisture ratio (MR) of 0.2 and 0.1. Under this level of MR, the pulsed electric field (PEF) and resting pretreatment exerts a protective effect of the tissue for both drying methods. However, under complete dehydration (water activity, aw = 0.05) damage seems to be similar for both drying methods despite the PEF pretreatment. Remarkably, reversible electroporation followed by resting resulted in higher trichome preservation. At MR of 0.05, the area of trichomes on the surface of convective-dried, PEF-rested and fresh samples were not statistically different at 2267 ± 89 µm2 and 2218 ± 65 µm2, respectively, showing that this pretreatment still exerts a protective effect on trichomes when complete dehydration is achieved.

(Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Drying methods, Pulsed electric field, Stress response, Thai basil, Trichomes integrity
in
Applied Sciences (Switzerland)
volume
12
issue
5
article number
2343
publisher
MDPI AG
external identifiers
  • scopus:85125306636
ISSN
2076-3417
DOI
10.3390/app12052343
project
Advancing optical microscopy for materials engineering in Lund University
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
id
06468c48-bf3b-48d5-9eaa-9a8326ea6529
date added to LUP
2022-03-20 17:59:16
date last changed
2023-12-09 20:03:10
@article{06468c48-bf3b-48d5-9eaa-9a8326ea6529,
  abstract     = {{<p>Pretreatment by reversible electroporation followed by resting (storage under saturated moisture at 21 ± 2 °C) was evaluated for modification of the properties of dried and rehydrated Thai basil leaves. The treated leaves were dried by convection at 40 °C or in a vacuum at room temperature. The results showed that vacuum drying provoked more cell damage and tissue collapse than convective air drying at a moisture ratio (MR) of 0.2 and 0.1. Under this level of MR, the pulsed electric field (PEF) and resting pretreatment exerts a protective effect of the tissue for both drying methods. However, under complete dehydration (water activity, aw = 0.05) damage seems to be similar for both drying methods despite the PEF pretreatment. Remarkably, reversible electroporation followed by resting resulted in higher trichome preservation. At MR of 0.05, the area of trichomes on the surface of convective-dried, PEF-rested and fresh samples were not statistically different at 2267 ± 89 µm<sup>2</sup> and 2218 ± 65 µm<sup>2</sup>, respectively, showing that this pretreatment still exerts a protective effect on trichomes when complete dehydration is achieved.</p>}},
  author       = {{Thamkaew, Grant and Rasmusson, Allan G. and Orlov, Dmytro and Galindo, Federico Gómez}},
  issn         = {{2076-3417}},
  keywords     = {{Drying methods; Pulsed electric field; Stress response; Thai basil; Trichomes integrity}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{5}},
  publisher    = {{MDPI AG}},
  series       = {{Applied Sciences (Switzerland)}},
  title        = {{Reversible Electroporation and Post-Electroporation Resting of Thai Basil Leaves Prior to Convective and Vacuum Drying}},
  url          = {{http://dx.doi.org/10.3390/app12052343}},
  doi          = {{10.3390/app12052343}},
  volume       = {{12}},
  year         = {{2022}},
}