Rapid multinodal acoustic trapping of extracellular vesicles for downstream mass spectrometry analysis
(2020) 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences p.148-149- Abstract
We report the use of multinodal acoustic trapping for high throughput and high capacity capturing of EV's (extracellular vesicles) for quantitative mass spectrometry analysis. The multinode trapping unit was shown to isolate sufficient amount of EV's from dilute biological samples (urine and cell culture supernatant) at flow rates of 500 ul/min within minutes, enabling EV proteome profiling. This was shown by differential protein expression analysis of urine and the urine EV fraction. Differential protein profiling of trapped EVs from stimulated versus non-stimulated platelets also demonstrated an easy access to differential expression in the EV-proteome.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/23616296-91cf-4443-9bd1-4aa7a51c3ab2
- author
- Broman, Axel LU ; Happonen, Lotta LU ; Palm, Frida LU ; Shannon, Oonagh LU ; Malmström, Johan LU and Laurell, Thomas LU
- organization
- publishing date
- 2020
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- series title
- MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
- conference location
- Virtual, Online
- conference dates
- 2020-10-04 - 2020-10-09
- external identifiers
-
- scopus:85098257338
- ISBN
- 9781733419017
- language
- English
- LU publication?
- yes
- id
- 23616296-91cf-4443-9bd1-4aa7a51c3ab2
- date added to LUP
- 2021-01-11 10:06:58
- date last changed
- 2023-09-24 18:41:11
@inproceedings{23616296-91cf-4443-9bd1-4aa7a51c3ab2, abstract = {{<p>We report the use of multinodal acoustic trapping for high throughput and high capacity capturing of EV's (extracellular vesicles) for quantitative mass spectrometry analysis. The multinode trapping unit was shown to isolate sufficient amount of EV's from dilute biological samples (urine and cell culture supernatant) at flow rates of 500 ul/min within minutes, enabling EV proteome profiling. This was shown by differential protein expression analysis of urine and the urine EV fraction. Differential protein profiling of trapped EVs from stimulated versus non-stimulated platelets also demonstrated an easy access to differential expression in the EV-proteome.</p>}}, author = {{Broman, Axel and Happonen, Lotta and Palm, Frida and Shannon, Oonagh and Malmström, Johan and Laurell, Thomas}}, booktitle = {{MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences}}, isbn = {{9781733419017}}, language = {{eng}}, pages = {{148--149}}, publisher = {{Chemical and Biological Microsystems Society}}, series = {{MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences}}, title = {{Rapid multinodal acoustic trapping of extracellular vesicles for downstream mass spectrometry analysis}}, year = {{2020}}, }