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Electrospray sample injection for single-particle imaging with x-ray lasers

Bielecki, Johan ; Hantke, Max F. ; Daurer, Benedikt J. ; Reddy, Hemanth K.N. ; Hasse, Dirk ; Larsson, Daniel S.D. ; Gunn, Laura H. ; Svenda, Martin ; Munke, Anna LU orcid and Sellberg, Jonas A. , et al. (2019) In Science Advances 5(5).
Abstract

The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with... (More)

The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
electrospray, XFEL, sample injection, X-ray free-electron laser (XFEL), free-electron laser, coherent diffraction imaging, single-particle imaging
in
Science Advances
volume
5
issue
5
article number
eaav8801
publisher
American Association for the Advancement of Science (AAAS)
external identifiers
  • scopus:85065606358
  • pmid:31058226
ISSN
2375-2548
DOI
10.1126/sciadv.aav8801
language
English
LU publication?
no
additional info
Publisher Copyright: © 2019 The Authors.
id
68dbc3a3-1983-4013-b024-3525cd3f5e5c
date added to LUP
2025-04-21 20:52:27
date last changed
2025-05-19 23:55:19
@article{68dbc3a3-1983-4013-b024-3525cd3f5e5c,
  abstract     = {{<p>The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.</p>}},
  author       = {{Bielecki, Johan and Hantke, Max F. and Daurer, Benedikt J. and Reddy, Hemanth K.N. and Hasse, Dirk and Larsson, Daniel S.D. and Gunn, Laura H. and Svenda, Martin and Munke, Anna and Sellberg, Jonas A. and Flueckiger, Leonie and Pietrini, Alberto and Nettelblad, Carl and Lundholm, Ida and Carlsson, Gunilla and Okamoto, Kenta and Timneanu, Nicusor and Westphal, Daniel and Kulyk, Olena and Higashiura, Akifumi and van der Schot, Gijs and Duane Loh, Ne Te and Wysong, Taylor E. and Bostedt, Christoph and Gorkhover, Tais and Iwan, Bianca and Seibert, M. Marvin and Osipov, Timur and Walter, Peter and Hart, Philip and Bucher, Maximilian and Ulmer, Anatoli and Ray, Dipanwita and Carini, Gabriella and Ferguson, Ken R. and Andersson, Inger and Andreasson, Jakob and Hajdu, Janos and Maia, Filipe R.N.C.}},
  issn         = {{2375-2548}},
  keywords     = {{electrospray; XFEL; sample injection; X-ray free-electron laser (XFEL); free-electron laser; coherent diffraction imaging; single-particle imaging}},
  language     = {{eng}},
  number       = {{5}},
  publisher    = {{American Association for the Advancement of Science (AAAS)}},
  series       = {{Science Advances}},
  title        = {{Electrospray sample injection for single-particle imaging with x-ray lasers}},
  url          = {{http://dx.doi.org/10.1126/sciadv.aav8801}},
  doi          = {{10.1126/sciadv.aav8801}},
  volume       = {{5}},
  year         = {{2019}},
}