Antiinflammatory and Anticancer Properties of Grewia asiatica Crude Extracts and Fractions : A Bioassay-Guided Approach
Qamar, Muhammad ; Akhtar, Saeed ; Barnard, Ross T. ; Sestili, Piero ; Ziora, Zyta M. ; Lazarte, Claudia E. LU and Ismail, Tariq LU
(2022)
In BioMed Research International
2022.
- Abstract
The study was an extension of our earlier work on antiinflammatory and anticancer properties of G. asiatica fruit. We aimed to develop a bioassay guided multistep purification technique for producing bioactive fractions of G. asiatica crude extracts. Dried fruit powder was sequentially fractionated with 100% dichloromethane, 100% methanol (MeOH), and 50% MeOH. Active extracts were subjected to liquid-liquid partitioning followed by subfractionation using RP-HPLC. Antioxidant, antiinflammatory, and anticancer activities of the fruit extracts, and their potent fractions were evaluated in vitro, while identification of compounds from the bioactive fractions was performed by ESI-MS/MS analysis. The amount of the... (More)
The study was an extension of our earlier work on antiinflammatory and anticancer properties of G. asiatica fruit. We aimed to develop a bioassay guided multistep purification technique for producing bioactive fractions of G. asiatica crude extracts. Dried fruit powder was sequentially fractionated with 100% dichloromethane, 100% methanol (MeOH), and 50% MeOH. Active extracts were subjected to liquid-liquid partitioning followed by subfractionation using RP-HPLC. Antioxidant, antiinflammatory, and anticancer activities of the fruit extracts, and their potent fractions were evaluated in vitro, while identification of compounds from the bioactive fractions was performed by ESI-MS/MS analysis. The amount of the identified compounds present was confirmed using external standards adopting a simple, accurate, and rapid analytical HPLC method. The results showed that 100% and 50% MeOH extracts possessed bioactivity; one of which (the 50% MeOH extract) displayed potent activity in all in vitro bioassays. MeOH extract (50%) derived fraction C and hydroalcoholic fraction 5 (GAHAF5) were observed to possess higher antioxidant, antiinflammatory, and in vitro anticancer activity. IC50 of GAHAF5 against MCF-7, HEp-2, and NCI-H522 cancer cells was recorded as 26.2, 51.4, and 63 μg/mL, respectively. ESI-MS/MS and HPLC analysis identified catechin, chlorogenic acid, caffeic acid, and morin as potential bioactive compounds in the GAHAF5 fraction with concentrations of 1230, 491, 957, and 130 μg/g, respectively. The findings indicated that G. asiatica bioactive fractions possessed antiinflammatory activity in vitro and were cytotoxic against breast cancer, lung cancer, and laryngeal cancer cell lines.
(Less)
- author
- Qamar, Muhammad
; Akhtar, Saeed
; Barnard, Ross T.
; Sestili, Piero
; Ziora, Zyta M.
; Lazarte, Claudia E.
LU
and Ismail, Tariq
LU
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- in
- BioMed Research International
- volume
- 2022
- article number
- 2277417
- pages
- 14 pages
- publisher
- Hindawi Limited
- external identifiers
-
- pmid:35386306
- scopus:85127621239
- ISSN
- 2314-6133
- DOI
- 10.1155/2022/2277417
- language
- English
- LU publication?
- yes
- id
- ce09b09f-1eea-4d62-9713-256c10bf86db
- date added to LUP
- 2022-06-10 12:08:10
- date last changed
- 2024-07-11 19:55:22
@article{ce09b09f-1eea-4d62-9713-256c10bf86db,
abstract = {{<p>The study was an extension of our earlier work on antiinflammatory and anticancer properties of <i>G. asiatica</i> fruit. We aimed to develop a bioassay guided multistep purification technique for producing bioactive fractions of <i>G. asiatica</i> crude extracts. Dried fruit powder was sequentially fractionated with 100% dichloromethane, 100% methanol (MeOH), and 50% MeOH. Active extracts were subjected to liquid-liquid partitioning followed by subfractionation using RP-HPLC. Antioxidant, antiinflammatory, and anticancer activities of the fruit extracts, and their potent fractions were evaluated <i>in vitro</i>, while identification of compounds from the bioactive fractions was performed by ESI-MS/MS analysis. The amount of the identified compounds present was confirmed using external standards adopting a simple, accurate, and rapid analytical HPLC method. The results showed that 100% and 50% MeOH extracts possessed bioactivity; one of which (the 50% MeOH extract) displayed potent activity in all <i>in vitro</i> bioassays. MeOH extract (50%) derived fraction C and hydroalcoholic fraction 5 (GAHAF5) were observed to possess higher antioxidant, antiinflammatory, and <i>in vitro</i> anticancer activity. IC50 of GAHAF5 against MCF-7, HEp-2, and NCI-H522 cancer cells was recorded as 26.2, 51.4, and 63 μg/mL, respectively. ESI-MS/MS and HPLC analysis identified catechin, chlorogenic acid, caffeic acid, and morin as potential bioactive compounds in the GAHAF5 fraction with concentrations of 1230, 491, 957, and 130 μg/g, respectively. The findings indicated that <i>G. asiatica</i> bioactive fractions possessed antiinflammatory activity <i>in vitro </i>and were cytotoxic against breast cancer, lung cancer, and laryngeal cancer cell lines. </p>}},
author = {{Qamar, Muhammad and Akhtar, Saeed and Barnard, Ross T. and Sestili, Piero and Ziora, Zyta M. and Lazarte, Claudia E. and Ismail, Tariq}},
issn = {{2314-6133}},
language = {{eng}},
publisher = {{Hindawi Limited}},
series = {{BioMed Research International}},
title = {{Antiinflammatory and Anticancer Properties of <i>Grewia asiatica</i> Crude Extracts and Fractions : A Bioassay-Guided Approach}},
url = {{http://dx.doi.org/10.1155/2022/2277417}},
doi = {{10.1155/2022/2277417}},
volume = {{2022}},
year = {{2022}},
}