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Biomarkers after Controlled Inhalation Exposure to Exhaust from Hydrogenated Vegetable Oil (HVO)

Krais, Annette M. LU orcid ; Essig, Julie Y. ; Gren, Louise LU ; Vogs, Carolina ; Assarsson, Eva LU ; Dierschke, Katrin LU ; Nielsen, Jörn LU ; Strandberg, Bo LU ; Pagels, Joakim LU and Broberg, Karin LU orcid , et al. (2021) In International Journal of Environmental Research and Public Health 18(12).
Abstract

Hydrogenated vegetable oil (HVO) is a renewable diesel fuel used to replace petroleum diesel. The organic compounds in HVO are poorly characterized; therefore, toxicological properties could be different from petroleum diesel exhaust. The aim of this study was to evaluate the exposure and effective biomarkers in 18 individuals after short-term (3 h) exposure to HVO exhaust and petroleum diesel exhaust fumes. Liquid chromatography tandem mass spectrometry was used to analyze urinary biomarkers. A proximity extension assay was used for the measurement of inflammatory proteins in plasma samples. Short-term (3 h) exposure to HVO exhaust (PM1 ~1 µg/m3 and ~90 µg/m3 for vehicles with and without exhaust... (More)

Hydrogenated vegetable oil (HVO) is a renewable diesel fuel used to replace petroleum diesel. The organic compounds in HVO are poorly characterized; therefore, toxicological properties could be different from petroleum diesel exhaust. The aim of this study was to evaluate the exposure and effective biomarkers in 18 individuals after short-term (3 h) exposure to HVO exhaust and petroleum diesel exhaust fumes. Liquid chromatography tandem mass spectrometry was used to analyze urinary biomarkers. A proximity extension assay was used for the measurement of inflammatory proteins in plasma samples. Short-term (3 h) exposure to HVO exhaust (PM1 ~1 µg/m3 and ~90 µg/m3 for vehicles with and without exhaust aftertreatment systems, respectively) did not increase any exposure biomarker, whereas petroleum diesel exhaust (PM1 ~300 µg/m3 ) increased urinary 4-MHA, a biomarker for p-xylene. HVO exhaust from the vehicle without exhaust aftertreatment system increased urinary 4-HNE-MA, a biomarker for lipid peroxidation, from 64 ng/mL urine (before exposure) to 141 ng/mL (24 h after exposure, p < 0.001). There was no differential expression of plasma inflammatory proteins between the HVO exhaust and control exposure group. In conclusion, short-term exposure to low concentrations of HVO exhaust did not increase urinary exposure biomarkers, but caused a slight increase in lipid peroxidation associated with the particle fraction.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Aerosol, Biomarkers, Exposure studies, HVO, Lipid peroxidation, Renewable diesel
in
International Journal of Environmental Research and Public Health
volume
18
issue
12
article number
6492
publisher
MDPI AG
external identifiers
  • scopus:85107893113
  • pmid:34208511
ISSN
1661-7827
DOI
10.3390/ijerph18126492
language
English
LU publication?
yes
id
d6b73ed2-8095-42fc-a401-03209f984652
date added to LUP
2021-06-28 13:32:15
date last changed
2024-06-29 14:06:52
@article{d6b73ed2-8095-42fc-a401-03209f984652,
  abstract     = {{<p>Hydrogenated vegetable oil (HVO) is a renewable diesel fuel used to replace petroleum diesel. The organic compounds in HVO are poorly characterized; therefore, toxicological properties could be different from petroleum diesel exhaust. The aim of this study was to evaluate the exposure and effective biomarkers in 18 individuals after short-term (3 h) exposure to HVO exhaust and petroleum diesel exhaust fumes. Liquid chromatography tandem mass spectrometry was used to analyze urinary biomarkers. A proximity extension assay was used for the measurement of inflammatory proteins in plasma samples. Short-term (3 h) exposure to HVO exhaust (PM<sub>1</sub> ~1 µg/m<sup>3</sup> and ~90 µg/m<sup>3</sup> for vehicles with and without exhaust aftertreatment systems, respectively) did not increase any exposure biomarker, whereas petroleum diesel exhaust (PM<sub>1</sub> ~300 µg/m<sup>3</sup> ) increased urinary 4-MHA, a biomarker for p-xylene. HVO exhaust from the vehicle without exhaust aftertreatment system increased urinary 4-HNE-MA, a biomarker for lipid peroxidation, from 64 ng/mL urine (before exposure) to 141 ng/mL (24 h after exposure, p &lt; 0.001). There was no differential expression of plasma inflammatory proteins between the HVO exhaust and control exposure group. In conclusion, short-term exposure to low concentrations of HVO exhaust did not increase urinary exposure biomarkers, but caused a slight increase in lipid peroxidation associated with the particle fraction.</p>}},
  author       = {{Krais, Annette M. and Essig, Julie Y. and Gren, Louise and Vogs, Carolina and Assarsson, Eva and Dierschke, Katrin and Nielsen, Jörn and Strandberg, Bo and Pagels, Joakim and Broberg, Karin and Lindh, Christian H. and Gudmundsson, Anders and Wierzbicka, Aneta}},
  issn         = {{1661-7827}},
  keywords     = {{Aerosol; Biomarkers; Exposure studies; HVO; Lipid peroxidation; Renewable diesel}},
  language     = {{eng}},
  month        = {{06}},
  number       = {{12}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Environmental Research and Public Health}},
  title        = {{Biomarkers after Controlled Inhalation Exposure to Exhaust from Hydrogenated Vegetable Oil (HVO)}},
  url          = {{http://dx.doi.org/10.3390/ijerph18126492}},
  doi          = {{10.3390/ijerph18126492}},
  volume       = {{18}},
  year         = {{2021}},
}