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Correlation of mitochondrial respiration in platelets, peripheral blood mononuclear cells and muscle fibers

Westerlund, Emil LU orcid ; Marelsson, Sigurður E. LU ; Karlsson, Michael ; Sjövall, Fredrik LU orcid ; Chamkha, Imen LU ; Åsander Frostner, Eleonor LU orcid ; Lundgren, Johan LU ; Fellman, Vineta LU orcid ; Eklund, Erik A. LU and Steding-Ehrenborg, Katarina LU , et al. (2024) In Heliyon 10(5).
Abstract

There is a growing interest for the possibility of using peripheral blood cells (including platelets) as markers for mitochondrial function in less accessible tissues. Only a few studies have examined the correlation between respiration in blood and muscle tissue, with small sample sizes and conflicting results. This study investigated the correlation of mitochondrial respiration within and across tissues. Additional analyses were performed to elucidate which blood cell type would be most useful for assessing systemic mitochondrial function. There was a significant but weak within tissue correlation between platelets and peripheral blood mononuclear cells (PBMCs). Neither PBMCs nor platelet respiration correlated significantly with... (More)

There is a growing interest for the possibility of using peripheral blood cells (including platelets) as markers for mitochondrial function in less accessible tissues. Only a few studies have examined the correlation between respiration in blood and muscle tissue, with small sample sizes and conflicting results. This study investigated the correlation of mitochondrial respiration within and across tissues. Additional analyses were performed to elucidate which blood cell type would be most useful for assessing systemic mitochondrial function. There was a significant but weak within tissue correlation between platelets and peripheral blood mononuclear cells (PBMCs). Neither PBMCs nor platelet respiration correlated significantly with muscle respiration. Muscle fibers from a group of athletes had higher mass-specific respiration, due to higher mitochondrial content than non-athlete controls, but this finding was not replicated in either of the blood cell types. In a group of patients with primary mitochondrial diseases, there were significant differences in blood cell respiration compared to healthy controls, particularly in platelets. Platelet respiration generally correlated better with the citrate synthase activity of each sample, in comparison to PBMCs. In conclusion, this study does not support the theory that blood cells can be used as accurate biomarkers to detect minor alterations in muscle respiration. However, in some instances, pronounced mitochondrial abnormalities might be reflected across tissues and detectable in blood cells, with more promising findings for platelets than PBMCs.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biomarkers, Correlation, Mitochondria, Muscle, Platelets, PMBCs
in
Heliyon
volume
10
issue
5
article number
e26745
publisher
Elsevier
external identifiers
  • pmid:38439844
  • scopus:85188210844
ISSN
2405-8440
DOI
10.1016/j.heliyon.2024.e26745
project
Development of treatment for mitochondrial disorders
language
English
LU publication?
yes
id
0d0a4dba-78b2-4aa3-b3b5-669e601b6bea
date added to LUP
2024-04-04 09:49:53
date last changed
2024-04-18 12:03:21
@article{0d0a4dba-78b2-4aa3-b3b5-669e601b6bea,
  abstract     = {{<p>There is a growing interest for the possibility of using peripheral blood cells (including platelets) as markers for mitochondrial function in less accessible tissues. Only a few studies have examined the correlation between respiration in blood and muscle tissue, with small sample sizes and conflicting results. This study investigated the correlation of mitochondrial respiration within and across tissues. Additional analyses were performed to elucidate which blood cell type would be most useful for assessing systemic mitochondrial function. There was a significant but weak within tissue correlation between platelets and peripheral blood mononuclear cells (PBMCs). Neither PBMCs nor platelet respiration correlated significantly with muscle respiration. Muscle fibers from a group of athletes had higher mass-specific respiration, due to higher mitochondrial content than non-athlete controls, but this finding was not replicated in either of the blood cell types. In a group of patients with primary mitochondrial diseases, there were significant differences in blood cell respiration compared to healthy controls, particularly in platelets. Platelet respiration generally correlated better with the citrate synthase activity of each sample, in comparison to PBMCs. In conclusion, this study does not support the theory that blood cells can be used as accurate biomarkers to detect minor alterations in muscle respiration. However, in some instances, pronounced mitochondrial abnormalities might be reflected across tissues and detectable in blood cells, with more promising findings for platelets than PBMCs.</p>}},
  author       = {{Westerlund, Emil and Marelsson, Sigurður E. and Karlsson, Michael and Sjövall, Fredrik and Chamkha, Imen and Åsander Frostner, Eleonor and Lundgren, Johan and Fellman, Vineta and Eklund, Erik A. and Steding-Ehrenborg, Katarina and Darin, Niklas and Paul, Gesine and Hansson, Magnus J. and Ehinger, Johannes K. and Elmér, Eskil}},
  issn         = {{2405-8440}},
  keywords     = {{Biomarkers; Correlation; Mitochondria; Muscle; Platelets; PMBCs}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{5}},
  publisher    = {{Elsevier}},
  series       = {{Heliyon}},
  title        = {{Correlation of mitochondrial respiration in platelets, peripheral blood mononuclear cells and muscle fibers}},
  url          = {{http://dx.doi.org/10.1016/j.heliyon.2024.e26745}},
  doi          = {{10.1016/j.heliyon.2024.e26745}},
  volume       = {{10}},
  year         = {{2024}},
}