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Immunolesioning of basal forebrain cholinergic neurons facilitates hippocampal kindling and perturbs neurotrophin messenger RNA regulation

Kokaia, Merab LU ; Ferencz, Istvan LU ; Leanza, G; Elmer, Eskil LU ; Metsis, M; Kokaia, Zaal LU ; Wiley, R G and Lindvall, Olle LU (1996) In Neuroscience 70(2). p.313-327
Abstract
The immunotoxin 192 IgG-saporin induces an efficient and specific lesion of low-affinity nerve growth factor receptor-bearing cholinergic neurons in the basal forebrain. Intraventricular injection of 192 IgG-saporin, which caused a complete loss of cholinergic afferents to the hippocampus and neocortex and a partial denervation of amygdala and piriform cortex, was found to markedly facilitate the initial stages of seizure development in hippocampal kindling. In contrast, the progression of kindling process from focal to generalized seizures was not affected. In situ hybridization demonstrated that basal levels of brain-derived neutrotrophic factor messenger RNA in the hippocampal formation and piriform cortex were significantly decreased... (More)
The immunotoxin 192 IgG-saporin induces an efficient and specific lesion of low-affinity nerve growth factor receptor-bearing cholinergic neurons in the basal forebrain. Intraventricular injection of 192 IgG-saporin, which caused a complete loss of cholinergic afferents to the hippocampus and neocortex and a partial denervation of amygdala and piriform cortex, was found to markedly facilitate the initial stages of seizure development in hippocampal kindling. In contrast, the progression of kindling process from focal to generalized seizures was not affected. In situ hybridization demonstrated that basal levels of brain-derived neutrotrophic factor messenger RNA in the hippocampal formation and piriform cortex were significantly decreased by the lesion, which also attenuated the seizure-induced increase of brain-derived neurotrophic factor messenger RNA expression in the hippocampus and frontal cortex. In the dentate gyrus, the 192 IgG-saporin lesion selectively reduced the upregulation of messenger RNAs for brain-derived neurotrophic factor exons I and III after a generalized seizure, whereas the increase of exon II messenger RNA was unchanged. The lesion abolished the seizure-evoked increase of nerve growth factor and TrkC messenger RNA levels and decrease of neutrophin-3 messenger RNA expression in dentate granule cells, while TrkB messenger RNA levels were not affected. We conclude that the basal forebrain cholinergic system (1) suppresses kindling epileptogenesis in the hippocampus, and (2) enhances both basal and seizure-evoked brain-derived neurotrophic factor synthesis in the hippocampal formation and some cortical areas through a specific pattern of activation of promoters within the brain-derived neurotrophic factor gene. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, cholinergic neurons, 192 IgG-saporin, kindling
in
Neuroscience
volume
70
issue
2
pages
313 - 327
publisher
Elsevier
external identifiers
  • pmid:8848142
ISSN
1873-7544
DOI
10.1016/0306-4522(95)00384-3
language
English
LU publication?
yes
id
f12525dc-9c17-4000-ba41-b6fdf935d197 (old id 1110548)
date added to LUP
2008-07-25 14:03:35
date last changed
2016-04-15 19:59:50
@article{f12525dc-9c17-4000-ba41-b6fdf935d197,
  abstract     = {The immunotoxin 192 IgG-saporin induces an efficient and specific lesion of low-affinity nerve growth factor receptor-bearing cholinergic neurons in the basal forebrain. Intraventricular injection of 192 IgG-saporin, which caused a complete loss of cholinergic afferents to the hippocampus and neocortex and a partial denervation of amygdala and piriform cortex, was found to markedly facilitate the initial stages of seizure development in hippocampal kindling. In contrast, the progression of kindling process from focal to generalized seizures was not affected. In situ hybridization demonstrated that basal levels of brain-derived neutrotrophic factor messenger RNA in the hippocampal formation and piriform cortex were significantly decreased by the lesion, which also attenuated the seizure-induced increase of brain-derived neurotrophic factor messenger RNA expression in the hippocampus and frontal cortex. In the dentate gyrus, the 192 IgG-saporin lesion selectively reduced the upregulation of messenger RNAs for brain-derived neurotrophic factor exons I and III after a generalized seizure, whereas the increase of exon II messenger RNA was unchanged. The lesion abolished the seizure-evoked increase of nerve growth factor and TrkC messenger RNA levels and decrease of neutrophin-3 messenger RNA expression in dentate granule cells, while TrkB messenger RNA levels were not affected. We conclude that the basal forebrain cholinergic system (1) suppresses kindling epileptogenesis in the hippocampus, and (2) enhances both basal and seizure-evoked brain-derived neurotrophic factor synthesis in the hippocampal formation and some cortical areas through a specific pattern of activation of promoters within the brain-derived neurotrophic factor gene.},
  author       = {Kokaia, Merab and Ferencz, Istvan and Leanza, G and Elmer, Eskil and Metsis, M and Kokaia, Zaal and Wiley, R G and Lindvall, Olle},
  issn         = {1873-7544},
  keyword      = {nerve growth factor,brain-derived neurotrophic factor,neurotrophin-3,cholinergic neurons,192 IgG-saporin,kindling},
  language     = {eng},
  number       = {2},
  pages        = {313--327},
  publisher    = {Elsevier},
  series       = {Neuroscience},
  title        = {Immunolesioning of basal forebrain cholinergic neurons facilitates hippocampal kindling and perturbs neurotrophin messenger RNA regulation},
  url          = {http://dx.doi.org/10.1016/0306-4522(95)00384-3},
  volume       = {70},
  year         = {1996},
}