Extracellular-Matrix-Reinforced Bioinks for 3D Bioprinting Human Tissue
(2021) In Advanced Materials 33(3).- Abstract
Recent advances in 3D bioprinting allow for generating intricate structures with dimensions relevant for human tissue, but suitable bioinks for producing translationally relevant tissue with complex geometries remain unidentified. Here, a tissue-specific hybrid bioink is described, composed of a natural polymer, alginate, reinforced with extracellular matrix derived from decellularized tissue (rECM). rECM has rheological and gelation properties beneficial for 3D bioprinting while retaining biologically inductive properties supporting tissue maturation ex vivo and in vivo. These bioinks are shear thinning, resist cell sedimentation, improve viability of multiple cell types, and enhance mechanical stability in hydrogels derived from them.... (More)
Recent advances in 3D bioprinting allow for generating intricate structures with dimensions relevant for human tissue, but suitable bioinks for producing translationally relevant tissue with complex geometries remain unidentified. Here, a tissue-specific hybrid bioink is described, composed of a natural polymer, alginate, reinforced with extracellular matrix derived from decellularized tissue (rECM). rECM has rheological and gelation properties beneficial for 3D bioprinting while retaining biologically inductive properties supporting tissue maturation ex vivo and in vivo. These bioinks are shear thinning, resist cell sedimentation, improve viability of multiple cell types, and enhance mechanical stability in hydrogels derived from them. 3D printed constructs generated from rECM bioinks suppress the foreign body response, are pro-angiogenic and support recipient-derived de novo blood vessel formation across the entire graft thickness in a murine model of transplant immunosuppression. Their proof-of-principle for generating human tissue is demonstrated by 3D bioprinting human airways composed of regionally specified primary human airway epithelial progenitor and smooth muscle cells. Airway lumens remained patent with viable cells for one month in vitro with evidence of differentiation into mature epithelial cell types found in native human airways. rECM bioinks are a promising new approach for generating functional human tissue using 3D bioprinting.
(Less)
- author
- organization
-
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- Lung Bioengineering and Regeneration (research group)
- WCMM-Wallenberg Centre for Molecular Medicine
- LUCC: Lund University Cancer Centre
- Stem Cell Center
- Molecular Vascular Physiology (research group)
- Department of Experimental Medical Science
- Cellular Biomechanics (research group)
- Molecular Skeletal Biology (research group)
- Clinical and experimental lung transplantation (research group)
- DCD transplantation of lungs (research group)
- Thoracic Surgery
- Childhood Cancer Research Unit (research group)
- Paediatrics (Lund)
- Division of Translational Cancer Research
- publishing date
- 2021
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Advanced Materials
- volume
- 33
- issue
- 3
- article number
- 2005476
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:33300242
- scopus:85097375646
- ISSN
- 1521-4095
- DOI
- 10.1002/adma.202005476
- language
- English
- LU publication?
- yes
- additional info
- © 2020 The Authors. Advanced Materials published by Wiley-VCH GmbH.
- id
- 127854eb-a77f-4df4-ba63-e5b0e52f0ca6
- date added to LUP
- 2020-12-12 19:23:53
- date last changed
- 2024-09-20 10:32:22
@article{127854eb-a77f-4df4-ba63-e5b0e52f0ca6, abstract = {{<p>Recent advances in 3D bioprinting allow for generating intricate structures with dimensions relevant for human tissue, but suitable bioinks for producing translationally relevant tissue with complex geometries remain unidentified. Here, a tissue-specific hybrid bioink is described, composed of a natural polymer, alginate, reinforced with extracellular matrix derived from decellularized tissue (rECM). rECM has rheological and gelation properties beneficial for 3D bioprinting while retaining biologically inductive properties supporting tissue maturation ex vivo and in vivo. These bioinks are shear thinning, resist cell sedimentation, improve viability of multiple cell types, and enhance mechanical stability in hydrogels derived from them. 3D printed constructs generated from rECM bioinks suppress the foreign body response, are pro-angiogenic and support recipient-derived de novo blood vessel formation across the entire graft thickness in a murine model of transplant immunosuppression. Their proof-of-principle for generating human tissue is demonstrated by 3D bioprinting human airways composed of regionally specified primary human airway epithelial progenitor and smooth muscle cells. Airway lumens remained patent with viable cells for one month in vitro with evidence of differentiation into mature epithelial cell types found in native human airways. rECM bioinks are a promising new approach for generating functional human tissue using 3D bioprinting.</p>}}, author = {{De Santis, Martina M and Alsafadi, Hani N and Tas, Sinem and Bölükbas, Deniz A and Prithiviraj, Sujeethkumar and Da Silva, Iran A and Mittendorfer, Margareta and Ota, Chiharu and Stegmayr, John and Daoud, Fatima and Königshoff, Melanie and Swärd, Karl and Wood, Jeffery A and Tassieri, Manlio and Bourgine, Paul E and Lindstedt, Sandra and Mohlin, Sofie and Wagner, Darcy E}}, issn = {{1521-4095}}, language = {{eng}}, number = {{3}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Advanced Materials}}, title = {{Extracellular-Matrix-Reinforced Bioinks for 3D Bioprinting Human Tissue}}, url = {{http://dx.doi.org/10.1002/adma.202005476}}, doi = {{10.1002/adma.202005476}}, volume = {{33}}, year = {{2021}}, }