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Rapid multinodal acoustic trapping of extracellular vesicles for downstream mass spectrometry analysis

Broman, Axel LU ; Happonen, Lotta LU ; Palm, Frida LU ; Shannon, Oonagh LU ; Malmström, Johan LU orcid and Laurell, Thomas LU (2020) 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 In MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences p.148-149
Abstract

We report the use of multinodal acoustic trapping for high throughput and high capacity capturing of EV's (extracellular vesicles) for quantitative mass spectrometry analysis. The multinode trapping unit was shown to isolate sufficient amount of EV's from dilute biological samples (urine and cell culture supernatant) at flow rates of 500 ul/min within minutes, enabling EV proteome profiling. This was shown by differential protein expression analysis of urine and the urine EV fraction. Differential protein profiling of trapped EVs from stimulated versus non-stimulated platelets also demonstrated an easy access to differential expression in the EV-proteome.

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author
; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
series title
MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
conference location
Virtual, Online
conference dates
2020-10-04 - 2020-10-09
external identifiers
  • scopus:85098257338
ISBN
9781733419017
language
English
LU publication?
yes
id
23616296-91cf-4443-9bd1-4aa7a51c3ab2
date added to LUP
2021-01-11 10:06:58
date last changed
2023-09-24 18:41:11
@inproceedings{23616296-91cf-4443-9bd1-4aa7a51c3ab2,
  abstract     = {{<p>We report the use of multinodal acoustic trapping for high throughput and high capacity capturing of EV's (extracellular vesicles) for quantitative mass spectrometry analysis. The multinode trapping unit was shown to isolate sufficient amount of EV's from dilute biological samples (urine and cell culture supernatant) at flow rates of 500 ul/min within minutes, enabling EV proteome profiling. This was shown by differential protein expression analysis of urine and the urine EV fraction. Differential protein profiling of trapped EVs from stimulated versus non-stimulated platelets also demonstrated an easy access to differential expression in the EV-proteome.</p>}},
  author       = {{Broman, Axel and Happonen, Lotta and Palm, Frida and Shannon, Oonagh and Malmström, Johan and Laurell, Thomas}},
  booktitle    = {{MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences}},
  isbn         = {{9781733419017}},
  language     = {{eng}},
  pages        = {{148--149}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  series       = {{MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences}},
  title        = {{Rapid multinodal acoustic trapping of extracellular vesicles for downstream mass spectrometry analysis}},
  year         = {{2020}},
}