Research Paper Impact of combining vitamin C with radiation therapy in human breast cancer : Does it matter?
(2022) In Oncotarget 13(1). p.439-453- Abstract
Vitamin C may impact the efficiency of radiation therapy (RT) in breast cancer. The effects of RT alone or in combination with vitamin C in SKBR3, MDA-MB-231, and MCF7 cells were compared using clonogenic assay, proliferation assay (MTT), cell cycle analysis, and Western blot. Vitamin C use was assessed in 1803 breast cancer patients 2002–2017 in relation to clinicopathological features and recurrences after RT. Vitamin C combined with RT resulted in non-significant increases in colony formation and minor differences in cell cycle arrest and expression of studied proteins, compared to RT alone. Lower vitamin C doses alone or in combination with RT, resulted in higher proliferation with MTT than higher vitamin C doses in a cell... (More)
Vitamin C may impact the efficiency of radiation therapy (RT) in breast cancer. The effects of RT alone or in combination with vitamin C in SKBR3, MDA-MB-231, and MCF7 cells were compared using clonogenic assay, proliferation assay (MTT), cell cycle analysis, and Western blot. Vitamin C use was assessed in 1803 breast cancer patients 2002–2017 in relation to clinicopathological features and recurrences after RT. Vitamin C combined with RT resulted in non-significant increases in colony formation and minor differences in cell cycle arrest and expression of studied proteins, compared to RT alone. Lower vitamin C doses alone or in combination with RT, resulted in higher proliferation with MTT than higher vitamin C doses in a cell line-dependent manner. Vitamin C use was associated with lower histological grade and BMI but not recurrence risk in RT-treated patients (LogRank P = 0.54). Vitamin C impacted RT efficiency differently depending on breast cancer subtype and vitamin C concentration. Lower doses of vitamin C, achievable with oral administration, might increase breast cancer cell proliferation and decrease radiosensitivity. Despite vitamin C users having less aggressive tumors than non-users, the recurrence risk in RT-treated patients was similar in vitamin C users and non-users.
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- author
- Khazaei, Somayeh LU ; Nilsson, Linn LU ; Adrian, Gabriel LU ; Tryggvadottir, Helga LU ; Konradsson, Elise LU ; Borgquist, Signe LU ; Isaksson, Karolin LU ; Ceberg, Crister LU and Jernström, Helena LU
- organization
-
- Division of Hematology and Transfusion Medicine
- LUCC: Lund University Cancer Centre
- Cancerepidemiology and radiation
- Radiation therapy
- Epidemiology and pharmacogenetics (research group)
- Medical Radiation Physics, Lund
- Radiotherapy Physics (research group)
- Breast cancer prevention & intervention (research group)
- Breastcancer
- Surgery (Lund)
- EpiHealth: Epidemiology for Health
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- clinical outcome, human breast cancer, in vitro, radiation therapy, vitamin C
- in
- Oncotarget
- volume
- 13
- issue
- 1
- pages
- 15 pages
- publisher
- Impact Journals
- external identifiers
-
- scopus:85127062809
- ISSN
- 1949-2553
- DOI
- 10.18632/ONCOTARGET.28204
- language
- English
- LU publication?
- yes
- id
- 270d579f-356b-4fe6-98a4-0a44478a7de7
- date added to LUP
- 2022-06-03 13:39:21
- date last changed
- 2024-05-16 18:15:03
@article{270d579f-356b-4fe6-98a4-0a44478a7de7, abstract = {{<p>Vitamin C may impact the efficiency of radiation therapy (RT) in breast cancer. The effects of RT alone or in combination with vitamin C in SKBR3, MDA-MB-231, and MCF7 cells were compared using clonogenic assay, proliferation assay (MTT), cell cycle analysis, and Western blot. Vitamin C use was assessed in 1803 breast cancer patients 2002–2017 in relation to clinicopathological features and recurrences after RT. Vitamin C combined with RT resulted in non-significant increases in colony formation and minor differences in cell cycle arrest and expression of studied proteins, compared to RT alone. Lower vitamin C doses alone or in combination with RT, resulted in higher proliferation with MTT than higher vitamin C doses in a cell line-dependent manner. Vitamin C use was associated with lower histological grade and BMI but not recurrence risk in RT-treated patients (LogRank P = 0.54). Vitamin C impacted RT efficiency differently depending on breast cancer subtype and vitamin C concentration. Lower doses of vitamin C, achievable with oral administration, might increase breast cancer cell proliferation and decrease radiosensitivity. Despite vitamin C users having less aggressive tumors than non-users, the recurrence risk in RT-treated patients was similar in vitamin C users and non-users.</p>}}, author = {{Khazaei, Somayeh and Nilsson, Linn and Adrian, Gabriel and Tryggvadottir, Helga and Konradsson, Elise and Borgquist, Signe and Isaksson, Karolin and Ceberg, Crister and Jernström, Helena}}, issn = {{1949-2553}}, keywords = {{clinical outcome; human breast cancer; in vitro; radiation therapy; vitamin C}}, language = {{eng}}, number = {{1}}, pages = {{439--453}}, publisher = {{Impact Journals}}, series = {{Oncotarget}}, title = {{Research Paper Impact of combining vitamin C with radiation therapy in human breast cancer : Does it matter?}}, url = {{http://dx.doi.org/10.18632/ONCOTARGET.28204}}, doi = {{10.18632/ONCOTARGET.28204}}, volume = {{13}}, year = {{2022}}, }