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Effect of surface and bulk solution properties on the adsorption of whey protein onto steel surfaces at high temperature

Santos, Olga LU ; Nylander, Tommy LU ; Schillén, Karin LU ; Paulsson, Marie LU and Trägårdh, Christian LU (2006) In Journal of Food Engineering 73(2). p.174-189
Abstract
The adsorption of whey proteins on modified and unmodified 316 2R stainless steel surfaces was studied by in situ ellipsometry under well-defined flow conditions. The effects of bulk properties, e.g. temperature, pH, protein concentration and flow rate as well as surface modification by ion implantation, sputtering, chemical vapour deposition and silica sol-gel deposition, were investigated. The surface properties were characterized in terms of wettability and roughness. The results suggest a surface-induced aggregation process given that (1) the adsorbed amount at high temperature was significantly larger than expected for a monolayer, and (2) the adsorption was lower with a larger number of aggregates in solution. The surface... (More)
The adsorption of whey proteins on modified and unmodified 316 2R stainless steel surfaces was studied by in situ ellipsometry under well-defined flow conditions. The effects of bulk properties, e.g. temperature, pH, protein concentration and flow rate as well as surface modification by ion implantation, sputtering, chemical vapour deposition and silica sol-gel deposition, were investigated. The surface properties were characterized in terms of wettability and roughness. The results suggest a surface-induced aggregation process given that (1) the adsorbed amount at high temperature was significantly larger than expected for a monolayer, and (2) the adsorption was lower with a larger number of aggregates in solution. The surface modifications affected the initial adsorption rate, i.e. the rate of forming the monolayer, which in turn influenced the surface induced aggregation and eventually the total amount deposited. Therefore, once the surface was fully covered with protein, the rate of deposition, which is controlled by protein-protein interactions, was less affected by the surface modifications. This work evidenced the potential of surface modification regarding the reduction of protein adsorption on steel surfaces. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
flow cell, ellipsometry, whey protein, adsorption, modified surfaces, dynamic light scattering, aggregation
in
Journal of Food Engineering
volume
73
issue
2
pages
174 - 189
publisher
Elsevier
external identifiers
  • wos:000233627300009
  • scopus:27144539180
ISSN
0260-8774
DOI
10.1016/j.jfoodeng.2005.01.018
language
English
LU publication?
yes
id
5b18d693-8281-4fb7-b038-40a1df9fa1f9 (old id 693883)
date added to LUP
2007-12-20 15:37:44
date last changed
2019-09-11 02:41:03
@article{5b18d693-8281-4fb7-b038-40a1df9fa1f9,
  abstract     = {The adsorption of whey proteins on modified and unmodified 316 2R stainless steel surfaces was studied by in situ ellipsometry under well-defined flow conditions. The effects of bulk properties, e.g. temperature, pH, protein concentration and flow rate as well as surface modification by ion implantation, sputtering, chemical vapour deposition and silica sol-gel deposition, were investigated. The surface properties were characterized in terms of wettability and roughness. The results suggest a surface-induced aggregation process given that (1) the adsorbed amount at high temperature was significantly larger than expected for a monolayer, and (2) the adsorption was lower with a larger number of aggregates in solution. The surface modifications affected the initial adsorption rate, i.e. the rate of forming the monolayer, which in turn influenced the surface induced aggregation and eventually the total amount deposited. Therefore, once the surface was fully covered with protein, the rate of deposition, which is controlled by protein-protein interactions, was less affected by the surface modifications. This work evidenced the potential of surface modification regarding the reduction of protein adsorption on steel surfaces.},
  author       = {Santos, Olga and Nylander, Tommy and Schillén, Karin and Paulsson, Marie and Trägårdh, Christian},
  issn         = {0260-8774},
  keyword      = {flow cell,ellipsometry,whey protein,adsorption,modified surfaces,dynamic light scattering,aggregation},
  language     = {eng},
  number       = {2},
  pages        = {174--189},
  publisher    = {Elsevier},
  series       = {Journal of Food Engineering},
  title        = {Effect of surface and bulk solution properties on the adsorption of whey protein onto steel surfaces at high temperature},
  url          = {http://dx.doi.org/10.1016/j.jfoodeng.2005.01.018},
  volume       = {73},
  year         = {2006},
}