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Xylanases and high-degree wet milling improve soluble dietary fibre content in liquid oat base

Mohammadi, Milad ; Norlander, Siri LU ; Hedström, Martin LU ; Adlercreutz, Patrick LU orcid and Grey, Carl LU (2024) In Food Chemistry 442.
Abstract

The growth of plant-based food and drink substitutes has led to increased interest in oat-based milk substitute as a dairy milk alternative. Conventional liquid oat base (LOB) production results in a fibre-rich insoluble by-product and loss of valuable macronutrients. This study investigates the use of xylanase enzymes to release insoluble arabinoxylan (AX) fibre and employs different degrees of milling in the LOB manufacturing process, with the aim to reduce insoluble waste and simultaneously increase soluble dietary fibre in oat-based milk substitutes. The combination of decreased mill gap space from 1 to 0.05 mm and addition of GH10 xylanase, resulted in a homogenous LOB product and solubilization of all available AX. Potential... (More)

The growth of plant-based food and drink substitutes has led to increased interest in oat-based milk substitute as a dairy milk alternative. Conventional liquid oat base (LOB) production results in a fibre-rich insoluble by-product and loss of valuable macronutrients. This study investigates the use of xylanase enzymes to release insoluble arabinoxylan (AX) fibre and employs different degrees of milling in the LOB manufacturing process, with the aim to reduce insoluble waste and simultaneously increase soluble dietary fibre in oat-based milk substitutes. The combination of decreased mill gap space from 1 to 0.05 mm and addition of GH10 xylanase, resulted in a homogenous LOB product and solubilization of all available AX. Potential prebiotic arabinoxylooligosaccharides of DP3-7 from GH10 hydrolysis were identified using HPAEC-PAD and MS analysis. These findings demonstrate the value of utilizing xylanases and fine-milling in LOB manufacturing, offering a sustainable approach to maximize health benefits of oat-based beverages.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Arabinoxylan, AXOS, Corundum mill, Enzymatic processing, Liquid oat base, Xylanase
in
Food Chemistry
volume
442
article number
138619
publisher
Elsevier
external identifiers
  • pmid:38306765
  • scopus:85183972588
ISSN
0308-8146
DOI
10.1016/j.foodchem.2024.138619
language
English
LU publication?
yes
id
861f6d2e-97e5-4d50-a26f-1bea69c67d0c
date added to LUP
2024-03-08 10:43:30
date last changed
2024-04-23 16:20:51
@article{861f6d2e-97e5-4d50-a26f-1bea69c67d0c,
  abstract     = {{<p>The growth of plant-based food and drink substitutes has led to increased interest in oat-based milk substitute as a dairy milk alternative. Conventional liquid oat base (LOB) production results in a fibre-rich insoluble by-product and loss of valuable macronutrients. This study investigates the use of xylanase enzymes to release insoluble arabinoxylan (AX) fibre and employs different degrees of milling in the LOB manufacturing process, with the aim to reduce insoluble waste and simultaneously increase soluble dietary fibre in oat-based milk substitutes. The combination of decreased mill gap space from 1 to 0.05 mm and addition of GH10 xylanase, resulted in a homogenous LOB product and solubilization of all available AX. Potential prebiotic arabinoxylooligosaccharides of DP3-7 from GH10 hydrolysis were identified using HPAEC-PAD and MS analysis. These findings demonstrate the value of utilizing xylanases and fine-milling in LOB manufacturing, offering a sustainable approach to maximize health benefits of oat-based beverages.</p>}},
  author       = {{Mohammadi, Milad and Norlander, Siri and Hedström, Martin and Adlercreutz, Patrick and Grey, Carl}},
  issn         = {{0308-8146}},
  keywords     = {{Arabinoxylan; AXOS; Corundum mill; Enzymatic processing; Liquid oat base; Xylanase}},
  language     = {{eng}},
  month        = {{06}},
  publisher    = {{Elsevier}},
  series       = {{Food Chemistry}},
  title        = {{Xylanases and high-degree wet milling improve soluble dietary fibre content in liquid oat base}},
  url          = {{http://dx.doi.org/10.1016/j.foodchem.2024.138619}},
  doi          = {{10.1016/j.foodchem.2024.138619}},
  volume       = {{442}},
  year         = {{2024}},
}