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Immune response and gut microbiota of mice on a diet mimicking eating habits of elderly with risk of malnutrition development

Håkansson, Åsa LU ; Nuur Farma Megaelectra, Afina LU ; Persson, Jenny ; Zhang, Su LU ; Alsanius, Beatrix ; Olsson, Marie and Jeppsson, Bengt LU (2021) In International Journal of Food Sciences and Nutrition 72(8). p.1071-1082
Abstract

The number of elderlies is increasing but prevalence of malnutrition has been reported. The aim of the study was to determine the significance of short-term nutritional deficiencies in mice. Immune status was assessed through flow cytometry of leucocytes in Peyer’s patches (PP) and mesenteric lymph nodes (MLN), and intestinal microbiota was evaluated by terminal restriction fragment length polymorphism (T-RFLP). C57BL/6NCrl mice fed standard diet (StD) or experimental diet high in fat, and low in carbohydrates, protein, fibre, vitamins, and minerals (ExpD) for 2 or 4 weeks. ExpD-animals gained less weight, increased liver lipids, and developed splenomegaly. Diet affected regulatory T-cells, gut homing receptors and TLR2 and TLR4 in PP... (More)

The number of elderlies is increasing but prevalence of malnutrition has been reported. The aim of the study was to determine the significance of short-term nutritional deficiencies in mice. Immune status was assessed through flow cytometry of leucocytes in Peyer’s patches (PP) and mesenteric lymph nodes (MLN), and intestinal microbiota was evaluated by terminal restriction fragment length polymorphism (T-RFLP). C57BL/6NCrl mice fed standard diet (StD) or experimental diet high in fat, and low in carbohydrates, protein, fibre, vitamins, and minerals (ExpD) for 2 or 4 weeks. ExpD-animals gained less weight, increased liver lipids, and developed splenomegaly. Diet affected regulatory T-cells, gut homing receptors and TLR2 and TLR4 in PP and MLN and the microbiota was influenced. Partial least squares models on flow cytometry- and T-RFLP data demonstrated correlations between microbial communities and immune phenotyping. Our model shows similarities to malnourished elderly and interactions between intestinal bacteria and the immune system.

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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Diet, elderly, immune responses, malnutrition, microbiota
in
International Journal of Food Sciences and Nutrition
volume
72
issue
8
pages
1071 - 1082
publisher
Taylor & Francis
external identifiers
  • pmid:33840335
  • scopus:85104324108
ISSN
0963-7486
DOI
10.1080/09637486.2021.1906212
language
English
LU publication?
yes
id
a48b2cc8-9b5e-4bdd-a012-0e07b498a81f
date added to LUP
2021-04-26 14:43:23
date last changed
2025-01-13 07:30:18
@article{a48b2cc8-9b5e-4bdd-a012-0e07b498a81f,
  abstract     = {{<p>The number of elderlies is increasing but prevalence of malnutrition has been reported. The aim of the study was to determine the significance of short-term nutritional deficiencies in mice. Immune status was assessed through flow cytometry of leucocytes in Peyer’s patches (PP) and mesenteric lymph nodes (MLN), and intestinal microbiota was evaluated by terminal restriction fragment length polymorphism (T-RFLP). C57BL/6NCrl mice fed standard diet (StD) or experimental diet high in fat, and low in carbohydrates, protein, fibre, vitamins, and minerals (ExpD) for 2 or 4 weeks. ExpD-animals gained less weight, increased liver lipids, and developed splenomegaly. Diet affected regulatory T-cells, gut homing receptors and TLR2 and TLR4 in PP and MLN and the microbiota was influenced. Partial least squares models on flow cytometry- and T-RFLP data demonstrated correlations between microbial communities and immune phenotyping. Our model shows similarities to malnourished elderly and interactions between intestinal bacteria and the immune system.</p>}},
  author       = {{Håkansson, Åsa and Nuur Farma Megaelectra, Afina and Persson, Jenny and Zhang, Su and Alsanius, Beatrix and Olsson, Marie and Jeppsson, Bengt}},
  issn         = {{0963-7486}},
  keywords     = {{Diet; elderly; immune responses; malnutrition; microbiota}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{1071--1082}},
  publisher    = {{Taylor & Francis}},
  series       = {{International Journal of Food Sciences and Nutrition}},
  title        = {{Immune response and gut microbiota of mice on a diet mimicking eating habits of elderly with risk of malnutrition development}},
  url          = {{http://dx.doi.org/10.1080/09637486.2021.1906212}},
  doi          = {{10.1080/09637486.2021.1906212}},
  volume       = {{72}},
  year         = {{2021}},
}