Exploring metabolic responses of potato tissue induced by electric pulses
Gomez, Federico LU ; Wadsö, Lars LU ; Vicente, A and Dejmek, Petr LU
(2008)
In Food Biophysics
- Abstract
- In this study, we investigated the metabolic responses of potato tissue induced by pulsed electric field (PEF). Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with a single rectangular pulse of 10 μs, 100 μs, or 1 ms. Metabolic responses were monitored using isothermal calorimetry, changes on electrical resistance during the delivery of the pulse, as well as impedance measurements. Our results show that the metabolic response involves oxygen consuming pathways as well as other unidentified events that are shown to be insensitive to metabolic inhibitors such as KCN and sodium azide. The metabolic response is strongly dependent on pulsing conditions and is independent of the total permeabilization achieved by the... (More)
- In this study, we investigated the metabolic responses of potato tissue induced by pulsed electric field (PEF). Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with a single rectangular pulse of 10 μs, 100 μs, or 1 ms. Metabolic responses were monitored using isothermal calorimetry, changes on electrical resistance during the delivery of the pulse, as well as impedance measurements. Our results show that the metabolic response involves oxygen consuming pathways as well as other unidentified events that are shown to be insensitive to metabolic inhibitors such as KCN and sodium azide. The metabolic response is strongly dependent on pulsing conditions and is independent of the total permeabilization achieved by the pulse. Evidence shows that calorimetry is a simple and powerful method for exploring conditions for metabolic stimulation, providing information on metabolic responses that can not be obtained from electrical measurements. This study set the basis for further investigations on defense-related consequences of PEF-induced stress. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1169697
- author
- Gomez, Federico
LU
; Wadsö, Lars
LU
; Vicente, A
and Dejmek, Petr
LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Metabolic stimulation, Isothermal calorimetry, Impedance, Electrical resistance
- in
- Food Biophysics
- publisher
- Springer
- external identifiers
-
- scopus:54249151917
- ISSN
- 1557-1866
- DOI
- 10.1007/s11483-008-9086-3
- language
- English
- LU publication?
- yes
- id
- eb1fd823-e637-44e1-b5d9-4ffceffc4222 (old id 1169697)
- date added to LUP
- 2016-04-01 12:20:27
- date last changed
- 2023-11-11 21:35:22
@article{eb1fd823-e637-44e1-b5d9-4ffceffc4222,
abstract = {{In this study, we investigated the metabolic responses of potato tissue induced by pulsed electric field (PEF). Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with a single rectangular pulse of 10 μs, 100 μs, or 1 ms. Metabolic responses were monitored using isothermal calorimetry, changes on electrical resistance during the delivery of the pulse, as well as impedance measurements. Our results show that the metabolic response involves oxygen consuming pathways as well as other unidentified events that are shown to be insensitive to metabolic inhibitors such as KCN and sodium azide. The metabolic response is strongly dependent on pulsing conditions and is independent of the total permeabilization achieved by the pulse. Evidence shows that calorimetry is a simple and powerful method for exploring conditions for metabolic stimulation, providing information on metabolic responses that can not be obtained from electrical measurements. This study set the basis for further investigations on defense-related consequences of PEF-induced stress.}},
author = {{Gomez, Federico and Wadsö, Lars and Vicente, A and Dejmek, Petr}},
issn = {{1557-1866}},
keywords = {{Metabolic stimulation; Isothermal calorimetry; Impedance; Electrical resistance}},
language = {{eng}},
publisher = {{Springer}},
series = {{Food Biophysics}},
title = {{Exploring metabolic responses of potato tissue induced by electric pulses}},
url = {{http://dx.doi.org/10.1007/s11483-008-9086-3}},
doi = {{10.1007/s11483-008-9086-3}},
year = {{2008}},
}