Proteomic changes to immune and inflammatory processes underlie lung preservation using ex vivo cytokine adsorption.
(2023) In Frontiers in Cardiovascular Medicine 10.- Abstract
INTRODUCTION: In recent years, the field of graft preservation has made considerable strides in improving outcomes related to solid organ restoration and regeneration. Ex vivo lung perfusion (EVLP) in line with the related devices and treatments has yielded promising results within preclinical and clinical studies, with the potential to improve graft quality. Its main benefit is to render marginal and declined donor lungs suitable for transplantation, ultimately increasing the donor pool available for transplantation. In addition, using such therapies in machine perfusion could also increase preservation time, facilitating logistical planning. Cytokine adsorption has been demonstrated as a potentially safe and effective therapy when... (More)
INTRODUCTION: In recent years, the field of graft preservation has made considerable strides in improving outcomes related to solid organ restoration and regeneration. Ex vivo lung perfusion (EVLP) in line with the related devices and treatments has yielded promising results within preclinical and clinical studies, with the potential to improve graft quality. Its main benefit is to render marginal and declined donor lungs suitable for transplantation, ultimately increasing the donor pool available for transplantation. In addition, using such therapies in machine perfusion could also increase preservation time, facilitating logistical planning. Cytokine adsorption has been demonstrated as a potentially safe and effective therapy when applied to the EVLP circuit and post-transplantation. However, the mechanism by which this therapy improves the donor lung on a molecular basis is not yet fully understood.
METHODS: We hypothesized that there were characteristic inflammatory and immunomodulatory differences between the lungs treated with and without cytokine adsorption, reflecting proteomic changes in the gene ontology pathways and across inflammation-related proteins. In this study, we investigate the molecular mechanisms and signaling pathways of how cytokine adsorption impacts lung function when used during EVLP and post-transplantation as hemoperfusion in a porcine model. Lung tissues during EVLP and post-lung transplantation were analyzed for their proteomic profiles using mass spectrometry.
RESULTS: We found through gene set enrichment analysis that the inflammatory and immune processes and coagulation pathways were significantly affected by the cytokine treatment after EVLP and transplantation.
CONCLUSION: In conclusion, we showed that the molecular mechanisms are using a proteomic approach behind the previously reported effects of cytokine adsorption when compared to the non-treated transplant recipients undergoing EVLP.
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- author
- Niroomand, Anna LU ; Hirdman, Gabriel LU ; Pierre, Leif LU ; Ghaidan, Haider LU ; Kjellström, Sven LU ; Stenlo, Martin LU ; Hyllén, Snejana LU ; Olm, Franziska LU and Lindstedt, Sandra LU
- organization
-
- Thoracic Surgery
- StemTherapy: National Initiative on Stem Cells for Regenerative Therapy
- Stem Cell Center
- WCMM-Wallenberg Centre for Molecular Medicine
- DCD transplantation of lungs (research group)
- BioMS (research group)
- Mass Spectrometry
- Anesthesiology and Intensive Care
- Clinical and experimental lung transplantation (research group)
- Cardiothoracic anesthesia and intensive care (research group)
- NPWT technology (research group)
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Frontiers in Cardiovascular Medicine
- volume
- 10
- article number
- 1274444
- publisher
- Frontiers Media S. A.
- external identifiers
-
- pmid:37849943
- scopus:85174305066
- ISSN
- 2297-055X
- DOI
- 10.3389/fcvm.2023.1274444
- language
- English
- LU publication?
- yes
- additional info
- © 2023 Niroomand, Hirdman, Pierre, Ghaidan, Kjellström, Stenlo, Hyllén, Olm and Lindstedt.
- id
- fa7ed1c2-f549-4a16-a025-eb2460515c11
- date added to LUP
- 2023-10-19 10:11:05
- date last changed
- 2024-09-18 14:17:45
@article{fa7ed1c2-f549-4a16-a025-eb2460515c11, abstract = {{<p>INTRODUCTION: In recent years, the field of graft preservation has made considerable strides in improving outcomes related to solid organ restoration and regeneration. Ex vivo lung perfusion (EVLP) in line with the related devices and treatments has yielded promising results within preclinical and clinical studies, with the potential to improve graft quality. Its main benefit is to render marginal and declined donor lungs suitable for transplantation, ultimately increasing the donor pool available for transplantation. In addition, using such therapies in machine perfusion could also increase preservation time, facilitating logistical planning. Cytokine adsorption has been demonstrated as a potentially safe and effective therapy when applied to the EVLP circuit and post-transplantation. However, the mechanism by which this therapy improves the donor lung on a molecular basis is not yet fully understood.</p><p>METHODS: We hypothesized that there were characteristic inflammatory and immunomodulatory differences between the lungs treated with and without cytokine adsorption, reflecting proteomic changes in the gene ontology pathways and across inflammation-related proteins. In this study, we investigate the molecular mechanisms and signaling pathways of how cytokine adsorption impacts lung function when used during EVLP and post-transplantation as hemoperfusion in a porcine model. Lung tissues during EVLP and post-lung transplantation were analyzed for their proteomic profiles using mass spectrometry.</p><p>RESULTS: We found through gene set enrichment analysis that the inflammatory and immune processes and coagulation pathways were significantly affected by the cytokine treatment after EVLP and transplantation.</p><p>CONCLUSION: In conclusion, we showed that the molecular mechanisms are using a proteomic approach behind the previously reported effects of cytokine adsorption when compared to the non-treated transplant recipients undergoing EVLP.</p>}}, author = {{Niroomand, Anna and Hirdman, Gabriel and Pierre, Leif and Ghaidan, Haider and Kjellström, Sven and Stenlo, Martin and Hyllén, Snejana and Olm, Franziska and Lindstedt, Sandra}}, issn = {{2297-055X}}, language = {{eng}}, publisher = {{Frontiers Media S. A.}}, series = {{Frontiers in Cardiovascular Medicine}}, title = {{Proteomic changes to immune and inflammatory processes underlie lung preservation using ex vivo cytokine adsorption.}}, url = {{http://dx.doi.org/10.3389/fcvm.2023.1274444}}, doi = {{10.3389/fcvm.2023.1274444}}, volume = {{10}}, year = {{2023}}, }