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Structure and morphology of vesicular dispersions based on novel glycophospholipids with various monosaccharide head groups

Barchan, Nikolina LU ; Gilbert, Jennifer LU orcid ; Pal, Antara LU ; Nylander, Tommy LU and Adlercreutz, Patrick LU orcid (2026) In Journal of Colloid and Interface Science 706.
Abstract

Glycophospholipids combine the structural versatility of phospholipids and carbohydrates, but their potential as excipients and performance in other related applications remains largely unexplored due to their low natural abundance. We have synthesized four novel phosphatidyl saccharide conjugates with different carbohydrate head groups; glucose, galactose, fructose and xylose by using a Phospholipase D catalysed transphosphatidylation reaction. The combination of Small Angle X-ray Scattering (SAXS) and Cryogenic Transmission Electron Microscopy (cryo-TEM) data allowed us to characterize the dispersed glycophospholipid vesicles in excess water and under physiologically relevant solution conditions in terms of their morphology and... (More)

Glycophospholipids combine the structural versatility of phospholipids and carbohydrates, but their potential as excipients and performance in other related applications remains largely unexplored due to their low natural abundance. We have synthesized four novel phosphatidyl saccharide conjugates with different carbohydrate head groups; glucose, galactose, fructose and xylose by using a Phospholipase D catalysed transphosphatidylation reaction. The combination of Small Angle X-ray Scattering (SAXS) and Cryogenic Transmission Electron Microscopy (cryo-TEM) data allowed us to characterize the dispersed glycophospholipid vesicles in excess water and under physiologically relevant solution conditions in terms of their morphology and structure. The different carbohydrate head group generated a large variability of the vesicle structures. Lipids conjugated with glucose and fructose self-assembled into unilamellar vesicles whereas galactose and xylose conjugated lipids formed multilamellar structures. Phosphatidylgalactose conjugated lipids formed a high number of stacked bilayers, while the phosphatidylxylose equivalent assembled into aggregates with only a few bilayers. These results highlight how carbohydrate hydroxyl spatial arrangements strongly influence lipid packing and self-assembly. The versatility of this glycophospholipid platform offers opportunities to generate biocompatible and biodegradable phospholipid excipients with properties that can be tailored for specific applications.

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Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bilayers, Cryo-TEM, Phospholipids, SAXS, Vesicles
in
Journal of Colloid and Interface Science
volume
706
article number
139585
pages
9 pages
publisher
Academic Press
external identifiers
  • scopus:105023950296
  • pmid:41352048
ISSN
0021-9797
DOI
10.1016/j.jcis.2025.139585
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2025 The Authors
id
1085dda3-f550-45b1-9452-ac2d6b345c02
date added to LUP
2025-12-19 14:19:21
date last changed
2025-12-23 03:08:22
@article{1085dda3-f550-45b1-9452-ac2d6b345c02,
  abstract     = {{<p>Glycophospholipids combine the structural versatility of phospholipids and carbohydrates, but their potential as excipients and performance in other related applications remains largely unexplored due to their low natural abundance. We have synthesized four novel phosphatidyl saccharide conjugates with different carbohydrate head groups; glucose, galactose, fructose and xylose by using a Phospholipase D catalysed transphosphatidylation reaction. The combination of Small Angle X-ray Scattering (SAXS) and Cryogenic Transmission Electron Microscopy (cryo-TEM) data allowed us to characterize the dispersed glycophospholipid vesicles in excess water and under physiologically relevant solution conditions in terms of their morphology and structure. The different carbohydrate head group generated a large variability of the vesicle structures. Lipids conjugated with glucose and fructose self-assembled into unilamellar vesicles whereas galactose and xylose conjugated lipids formed multilamellar structures. Phosphatidylgalactose conjugated lipids formed a high number of stacked bilayers, while the phosphatidylxylose equivalent assembled into aggregates with only a few bilayers. These results highlight how carbohydrate hydroxyl spatial arrangements strongly influence lipid packing and self-assembly. The versatility of this glycophospholipid platform offers opportunities to generate biocompatible and biodegradable phospholipid excipients with properties that can be tailored for specific applications.</p>}},
  author       = {{Barchan, Nikolina and Gilbert, Jennifer and Pal, Antara and Nylander, Tommy and Adlercreutz, Patrick}},
  issn         = {{0021-9797}},
  keywords     = {{Bilayers; Cryo-TEM; Phospholipids; SAXS; Vesicles}},
  language     = {{eng}},
  month        = {{03}},
  publisher    = {{Academic Press}},
  series       = {{Journal of Colloid and Interface Science}},
  title        = {{Structure and morphology of vesicular dispersions based on novel glycophospholipids with various monosaccharide head groups}},
  url          = {{http://dx.doi.org/10.1016/j.jcis.2025.139585}},
  doi          = {{10.1016/j.jcis.2025.139585}},
  volume       = {{706}},
  year         = {{2026}},
}