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Antiproliferative effects of sea buckthorn (Hippophae rhamnoides L.) extracts on human colon and liver cancer cell lines

Grey, Carl LU ; Widen, Cecilia; Adlercreutz, Patrick LU ; Rumpunen, Kimmo and Duan, Rui-Dong LU (2010) In Food Chemistry 120(4). p.1004-1010
Abstract
Sea buckthorn berries contain many bioactive compounds that have anticancer properties. To investigate whether the anti proliferative effects Could be associated with the presence of certain compounds. a sequential extraction was performed. The extraction started with heptane followed by ethyl acetate, ethanol, and water. A second protocol using ethanol:water (1:1) was also used. The contents of the extracts were determined and their effects on cell proliferation were investigated in both Caco-2 and Hep G2 cells. The ethyl acetate fraction was exclusively found to contain high levels of ursolic acid, together with low amounts of phenolics. The ethanol:water extracts contained high levels of phenolic compounds and proanthyocyanidin, but... (More)
Sea buckthorn berries contain many bioactive compounds that have anticancer properties. To investigate whether the anti proliferative effects Could be associated with the presence of certain compounds. a sequential extraction was performed. The extraction started with heptane followed by ethyl acetate, ethanol, and water. A second protocol using ethanol:water (1:1) was also used. The contents of the extracts were determined and their effects on cell proliferation were investigated in both Caco-2 and Hep G2 cells. The ethyl acetate fraction was exclusively found to contain high levels of ursolic acid, together with low amounts of phenolics. The ethanol:water extracts contained high levels of phenolic compounds and proanthyocyanidin, but little ursolic acid. When the antiproliferative effects were examined, the strongest inhibitory effect was found in the ethyl acetate extract for the Caco-2 cells and in the ethanol:water extract for the Hep G2 cells. The antiproliferative effects were in both cases dose-dependent and were in the case of the ethyl acetate extract associated with an increase in apoptosis. The results obtained show that the choice of extraction solvent is of considerable importance and that ursolic acid might be more important than the polyphenols in inhibiting the cancer cell proliferation. (C) 2009 Elsevier Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
acid, Ursolic, Sea buckthorn, Rutin, Proanthocyanidin, Phenols, Isorhamnetin, Hep G2 cells, Cell culture, Caco-2 cells, Antiproliferation, Apoptosis
in
Food Chemistry
volume
120
issue
4
pages
1004 - 1010
publisher
Elsevier
external identifiers
  • wos:000275010600008
  • scopus:75049085047
ISSN
1873-7072
DOI
10.1016/j.foodchem.2009.11.039
language
English
LU publication?
yes
id
cc07bb17-0830-497c-afd0-7f6bfd8dc6cb (old id 1589571)
date added to LUP
2010-04-20 10:47:37
date last changed
2018-05-29 10:02:09
@article{cc07bb17-0830-497c-afd0-7f6bfd8dc6cb,
  abstract     = {Sea buckthorn berries contain many bioactive compounds that have anticancer properties. To investigate whether the anti proliferative effects Could be associated with the presence of certain compounds. a sequential extraction was performed. The extraction started with heptane followed by ethyl acetate, ethanol, and water. A second protocol using ethanol:water (1:1) was also used. The contents of the extracts were determined and their effects on cell proliferation were investigated in both Caco-2 and Hep G2 cells. The ethyl acetate fraction was exclusively found to contain high levels of ursolic acid, together with low amounts of phenolics. The ethanol:water extracts contained high levels of phenolic compounds and proanthyocyanidin, but little ursolic acid. When the antiproliferative effects were examined, the strongest inhibitory effect was found in the ethyl acetate extract for the Caco-2 cells and in the ethanol:water extract for the Hep G2 cells. The antiproliferative effects were in both cases dose-dependent and were in the case of the ethyl acetate extract associated with an increase in apoptosis. The results obtained show that the choice of extraction solvent is of considerable importance and that ursolic acid might be more important than the polyphenols in inhibiting the cancer cell proliferation. (C) 2009 Elsevier Ltd. All rights reserved.},
  author       = {Grey, Carl and Widen, Cecilia and Adlercreutz, Patrick and Rumpunen, Kimmo and Duan, Rui-Dong},
  issn         = {1873-7072},
  keyword      = {acid,Ursolic,Sea buckthorn,Rutin,Proanthocyanidin,Phenols,Isorhamnetin,Hep G2 cells,Cell culture,Caco-2 cells,Antiproliferation,Apoptosis},
  language     = {eng},
  number       = {4},
  pages        = {1004--1010},
  publisher    = {Elsevier},
  series       = {Food Chemistry},
  title        = {Antiproliferative effects of sea buckthorn (Hippophae rhamnoides L.) extracts on human colon and liver cancer cell lines},
  url          = {http://dx.doi.org/10.1016/j.foodchem.2009.11.039},
  volume       = {120},
  year         = {2010},
}