Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Proteomic changes to immune and inflammatory processes underlie lung preservation using ex vivo cytokine adsorption.

Niroomand, Anna LU ; Hirdman, Gabriel LU ; Pierre, Leif LU ; Ghaidan, Haider LU orcid ; Kjellström, Sven LU ; Stenlo, Martin LU ; Hyllén, Snejana LU ; Olm, Franziska LU orcid and Lindstedt, Sandra LU (2023) In Frontiers in Cardiovascular Medicine 10.
Abstract

INTRODUCTION: In recent years, the field of graft preservation has made considerable strides in improving outcomes related to solid organ restoration and regeneration. Ex vivo lung perfusion (EVLP) in line with the related devices and treatments has yielded promising results within preclinical and clinical studies, with the potential to improve graft quality. Its main benefit is to render marginal and declined donor lungs suitable for transplantation, ultimately increasing the donor pool available for transplantation. In addition, using such therapies in machine perfusion could also increase preservation time, facilitating logistical planning. Cytokine adsorption has been demonstrated as a potentially safe and effective therapy when... (More)

INTRODUCTION: In recent years, the field of graft preservation has made considerable strides in improving outcomes related to solid organ restoration and regeneration. Ex vivo lung perfusion (EVLP) in line with the related devices and treatments has yielded promising results within preclinical and clinical studies, with the potential to improve graft quality. Its main benefit is to render marginal and declined donor lungs suitable for transplantation, ultimately increasing the donor pool available for transplantation. In addition, using such therapies in machine perfusion could also increase preservation time, facilitating logistical planning. Cytokine adsorption has been demonstrated as a potentially safe and effective therapy when applied to the EVLP circuit and post-transplantation. However, the mechanism by which this therapy improves the donor lung on a molecular basis is not yet fully understood.

METHODS: We hypothesized that there were characteristic inflammatory and immunomodulatory differences between the lungs treated with and without cytokine adsorption, reflecting proteomic changes in the gene ontology pathways and across inflammation-related proteins. In this study, we investigate the molecular mechanisms and signaling pathways of how cytokine adsorption impacts lung function when used during EVLP and post-transplantation as hemoperfusion in a porcine model. Lung tissues during EVLP and post-lung transplantation were analyzed for their proteomic profiles using mass spectrometry.

RESULTS: We found through gene set enrichment analysis that the inflammatory and immune processes and coagulation pathways were significantly affected by the cytokine treatment after EVLP and transplantation.

CONCLUSION: In conclusion, we showed that the molecular mechanisms are using a proteomic approach behind the previously reported effects of cytokine adsorption when compared to the non-treated transplant recipients undergoing EVLP.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Frontiers in Cardiovascular Medicine
volume
10
article number
1274444
publisher
Frontiers Media S. A.
external identifiers
  • scopus:85174305066
  • pmid:37849943
ISSN
2297-055X
DOI
10.3389/fcvm.2023.1274444
language
English
LU publication?
yes
additional info
© 2023 Niroomand, Hirdman, Pierre, Ghaidan, Kjellström, Stenlo, Hyllén, Olm and Lindstedt.
id
fa7ed1c2-f549-4a16-a025-eb2460515c11
date added to LUP
2023-10-19 10:11:05
date last changed
2024-04-16 23:52:28
@article{fa7ed1c2-f549-4a16-a025-eb2460515c11,
  abstract     = {{<p>INTRODUCTION: In recent years, the field of graft preservation has made considerable strides in improving outcomes related to solid organ restoration and regeneration. Ex vivo lung perfusion (EVLP) in line with the related devices and treatments has yielded promising results within preclinical and clinical studies, with the potential to improve graft quality. Its main benefit is to render marginal and declined donor lungs suitable for transplantation, ultimately increasing the donor pool available for transplantation. In addition, using such therapies in machine perfusion could also increase preservation time, facilitating logistical planning. Cytokine adsorption has been demonstrated as a potentially safe and effective therapy when applied to the EVLP circuit and post-transplantation. However, the mechanism by which this therapy improves the donor lung on a molecular basis is not yet fully understood.</p><p>METHODS: We hypothesized that there were characteristic inflammatory and immunomodulatory differences between the lungs treated with and without cytokine adsorption, reflecting proteomic changes in the gene ontology pathways and across inflammation-related proteins. In this study, we investigate the molecular mechanisms and signaling pathways of how cytokine adsorption impacts lung function when used during EVLP and post-transplantation as hemoperfusion in a porcine model. Lung tissues during EVLP and post-lung transplantation were analyzed for their proteomic profiles using mass spectrometry.</p><p>RESULTS: We found through gene set enrichment analysis that the inflammatory and immune processes and coagulation pathways were significantly affected by the cytokine treatment after EVLP and transplantation.</p><p>CONCLUSION: In conclusion, we showed that the molecular mechanisms are using a proteomic approach behind the previously reported effects of cytokine adsorption when compared to the non-treated transplant recipients undergoing EVLP.</p>}},
  author       = {{Niroomand, Anna and Hirdman, Gabriel and Pierre, Leif and Ghaidan, Haider and Kjellström, Sven and Stenlo, Martin and Hyllén, Snejana and Olm, Franziska and Lindstedt, Sandra}},
  issn         = {{2297-055X}},
  language     = {{eng}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Cardiovascular Medicine}},
  title        = {{Proteomic changes to immune and inflammatory processes underlie lung preservation using 
        ex vivo cytokine adsorption.}},
  url          = {{http://dx.doi.org/10.3389/fcvm.2023.1274444}},
  doi          = {{10.3389/fcvm.2023.1274444}},
  volume       = {{10}},
  year         = {{2023}},
}