Ex Vivo Working Porcine Heart Model
Pigot, Harry LU
; Soltesz, Kristian
LU
and Steen, Stig
LU
(2024)
In Methods in Molecular Biology
2803.
p.87-107
- Abstract
- Ex vivo working porcine heart models allow for the study of a heart’s function and physiology outside the living organism. These models are particularly useful due to the anatomical and physiological similarities between porcine and human hearts, providing an experimental platform to investigate cardiac disease or assess donor heart viability for transplantation. This chapter presents an in-depth discussion of the model’s components, including the perfusate, preload, and afterload. We explore the challenges of emulating cardiac afterload and present a historical perspective on afterload modeling, discussing various methodologies and their respective limitations. An actively controlled afterload device is introduced to enhance the model’s... (More)
- Ex vivo working porcine heart models allow for the study of a heart’s function and physiology outside the living organism. These models are particularly useful due to the anatomical and physiological similarities between porcine and human hearts, providing an experimental platform to investigate cardiac disease or assess donor heart viability for transplantation. This chapter presents an in-depth discussion of the model’s components, including the perfusate, preload, and afterload. We explore the challenges of emulating cardiac afterload and present a historical perspective on afterload modeling, discussing various methodologies and their respective limitations. An actively controlled afterload device is introduced to enhance the model’s ability to rapidly adjust pressure in the large arteries, thereby providing a more accurate and dynamic experimental model. Finally, we provide a comprehensive experimental protocol for the ex vivo working porcine heart model. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/0a288de4-1f77-40f6-a77c-3b3173b6861a
- author
- Pigot, Harry
LU
; Soltesz, Kristian
LU
and Steen, Stig
LU
- organization
- publishing date
- 2024-04-28
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Working porcine heart, Isolated heart preparation, Functional heart evaluation, cardiac afterload, cyber-physiological systems, functional heart assessment, ex vivo heart perfusion, ex situ heart perfusion, ex vivo heart evaluation, heart transplantation
- host publication
- Experimental Models of Cardiovascular Diseases : Methods and Protocols - Methods and Protocols
- series title
- Methods in Molecular Biology
- editor
- Ishikawa, Kiyotake
- volume
- 2803
- edition
- 2
- pages
- 20 pages
- publisher
- Humana Press
- external identifiers
-
- pmid:38676887
- scopus:85191639979
- ISSN
- 1064-3745
- 1940-6029
- ISBN
- 978-1-0716-3848-4
- 978-1-0716-3845-3
- 978-1-0716-3846-0
- DOI
- 10.1007/978-1-0716-3846-0_7
- project
- Functional ex vivo heart evaluation
- language
- English
- LU publication?
- yes
- id
- 0a288de4-1f77-40f6-a77c-3b3173b6861a
- date added to LUP
- 2024-05-06 16:24:29
- date last changed
- 2024-06-18 07:51:20
@inbook{0a288de4-1f77-40f6-a77c-3b3173b6861a,
abstract = {{Ex vivo working porcine heart models allow for the study of a heart’s function and physiology outside the living organism. These models are particularly useful due to the anatomical and physiological similarities between porcine and human hearts, providing an experimental platform to investigate cardiac disease or assess donor heart viability for transplantation. This chapter presents an in-depth discussion of the model’s components, including the perfusate, preload, and afterload. We explore the challenges of emulating cardiac afterload and present a historical perspective on afterload modeling, discussing various methodologies and their respective limitations. An actively controlled afterload device is introduced to enhance the model’s ability to rapidly adjust pressure in the large arteries, thereby providing a more accurate and dynamic experimental model. Finally, we provide a comprehensive experimental protocol for the ex vivo working porcine heart model.}},
author = {{Pigot, Harry and Soltesz, Kristian and Steen, Stig}},
booktitle = {{Experimental Models of Cardiovascular Diseases : Methods and Protocols}},
editor = {{Ishikawa, Kiyotake}},
isbn = {{978-1-0716-3848-4}},
issn = {{1064-3745}},
keywords = {{Working porcine heart; Isolated heart preparation; Functional heart evaluation; cardiac afterload; cyber-physiological systems; functional heart assessment; ex vivo heart perfusion; ex situ heart perfusion; ex vivo heart evaluation; heart transplantation}},
language = {{eng}},
month = {{04}},
pages = {{87--107}},
publisher = {{Humana Press}},
series = {{Methods in Molecular Biology}},
title = {{Ex Vivo Working Porcine Heart Model}},
url = {{http://dx.doi.org/10.1007/978-1-0716-3846-0_7}},
doi = {{10.1007/978-1-0716-3846-0_7}},
volume = {{2803}},
year = {{2024}},
}