Advanced

Survival of Lactobacillus plantarum DSM 9843 (299v), and effect on the short-chain fatty acid content of faeces after ingestion of a rose-hip drink with fermented oats

Johansson, M L LU ; Nobaek, S; Berggren, A; Nyman, M LU ; Björck, I LU ; Ahrné, S LU ; Jeppsson, B LU and Molin, G LU (1998) In International Journal of Food Microbiology 42(1-2). p.29-38
Abstract

In a controlled and randomised double-blind study, 26 healthy adult volunteers consumed, for 21 d, 400 ml of a rose-hip drink containing oats (0.7 g/100ml) fermented with Lactobacillus plantarum DSM 9843 (RHL; containing 5 x 10(7) cfu ml(-1)), and 22 volunteers in a second group the same amount of a pure rose-hip drink (RH). Significant increases in the total faecal concentration of carboxylic acids (P < 0.05 after 1 week and P < 0.01 after 3 weeks of intake), acetic acid (P < 0.01 after 3 weeks of intake) and propionic acid (P < 0.01 after 3 weeks of intake and P < 0.05 eight days after intake ceased) were recorded in the RHL group, indicating increased fermentation in the colon. In both groups a significant increase was... (More)

In a controlled and randomised double-blind study, 26 healthy adult volunteers consumed, for 21 d, 400 ml of a rose-hip drink containing oats (0.7 g/100ml) fermented with Lactobacillus plantarum DSM 9843 (RHL; containing 5 x 10(7) cfu ml(-1)), and 22 volunteers in a second group the same amount of a pure rose-hip drink (RH). Significant increases in the total faecal concentration of carboxylic acids (P < 0.05 after 1 week and P < 0.01 after 3 weeks of intake), acetic acid (P < 0.01 after 3 weeks of intake) and propionic acid (P < 0.01 after 3 weeks of intake and P < 0.05 eight days after intake ceased) were recorded in the RHL group, indicating increased fermentation in the colon. In both groups a significant increase was obtained in the concentration of faecal lactic acid (P < 0.001 after 1 and 3 weeks of intake). No changes were seen in the concentration of faecal butyrate. The numbers of faecal bifidobacteria and lactobacilli increased significantly in both groups after 3 weeks of intake. Sulphite-reducing clostridia rapidly decreased in the group receiving the product with Lb. plantarum DSM 9843 after 1 week of intake, and then also in the pure rose-hip group after 3 weeks of intake. No changes were seen in the numbers of total anaerobes, gram-negative anaerobes or total aerobes during administration. Lb. plantarum DSM 9843 was recovered in faeces from all volunteers in the RHL group. Median amounts were 7.0 (5.0-8.8) log10 cfu g(-1) after one week of intake, and 6.7 (5.0-8.9) log10 cfu g(-1) after 3 weeks, respectively. The strain was still recovered from faeces of five volunteers 8 d after administration ceased (> 4.8 log10 cfu g(-1)). During the period of intake the volunteers in the RHL group experienced a significant increase in stool volume, a significant decrease in flatulence and slightly softer stools. Volunteers in the RH group experienced a slight but significant decrease in stool volume.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adult, Avena, Beverages, Carboxylic Acids/analysis, Defecation/physiology, Double-Blind Method, Fatty Acids, Volatile/analysis, Feces/chemistry, Female, Fermentation, Humans, Hydrogen-Ion Concentration, Lactobacillus/isolation & purification, Male, Middle Aged, Probiotics
in
International Journal of Food Microbiology
volume
42
issue
1-2
pages
29 - 38
publisher
Elsevier
external identifiers
  • scopus:0032581102
ISSN
0168-1605
DOI
10.1016/S0168-1605(98)00055-5
language
English
LU publication?
yes
id
103ab787-a76c-4961-8255-70c2d02f556b
date added to LUP
2018-10-16 18:49:33
date last changed
2019-11-05 05:04:32
@article{103ab787-a76c-4961-8255-70c2d02f556b,
  abstract     = {<p>In a controlled and randomised double-blind study, 26 healthy adult volunteers consumed, for 21 d, 400 ml of a rose-hip drink containing oats (0.7 g/100ml) fermented with Lactobacillus plantarum DSM 9843 (RHL; containing 5 x 10(7) cfu ml(-1)), and 22 volunteers in a second group the same amount of a pure rose-hip drink (RH). Significant increases in the total faecal concentration of carboxylic acids (P &lt; 0.05 after 1 week and P &lt; 0.01 after 3 weeks of intake), acetic acid (P &lt; 0.01 after 3 weeks of intake) and propionic acid (P &lt; 0.01 after 3 weeks of intake and P &lt; 0.05 eight days after intake ceased) were recorded in the RHL group, indicating increased fermentation in the colon. In both groups a significant increase was obtained in the concentration of faecal lactic acid (P &lt; 0.001 after 1 and 3 weeks of intake). No changes were seen in the concentration of faecal butyrate. The numbers of faecal bifidobacteria and lactobacilli increased significantly in both groups after 3 weeks of intake. Sulphite-reducing clostridia rapidly decreased in the group receiving the product with Lb. plantarum DSM 9843 after 1 week of intake, and then also in the pure rose-hip group after 3 weeks of intake. No changes were seen in the numbers of total anaerobes, gram-negative anaerobes or total aerobes during administration. Lb. plantarum DSM 9843 was recovered in faeces from all volunteers in the RHL group. Median amounts were 7.0 (5.0-8.8) log10 cfu g(-1) after one week of intake, and 6.7 (5.0-8.9) log10 cfu g(-1) after 3 weeks, respectively. The strain was still recovered from faeces of five volunteers 8 d after administration ceased (&gt; 4.8 log10 cfu g(-1)). During the period of intake the volunteers in the RHL group experienced a significant increase in stool volume, a significant decrease in flatulence and slightly softer stools. Volunteers in the RH group experienced a slight but significant decrease in stool volume.</p>},
  author       = {Johansson, M L and Nobaek, S and Berggren, A and Nyman, M and Björck, I and Ahrné, S and Jeppsson, B and Molin, G},
  issn         = {0168-1605},
  keyword      = {Adult,Avena,Beverages,Carboxylic Acids/analysis,Defecation/physiology,Double-Blind Method,Fatty Acids, Volatile/analysis,Feces/chemistry,Female,Fermentation,Humans,Hydrogen-Ion Concentration,Lactobacillus/isolation & purification,Male,Middle Aged,Probiotics},
  language     = {eng},
  month        = {06},
  number       = {1-2},
  pages        = {29--38},
  publisher    = {Elsevier},
  series       = {International Journal of Food Microbiology},
  title        = {Survival of Lactobacillus plantarum DSM 9843 (299v), and effect on the short-chain fatty acid content of faeces after ingestion of a rose-hip drink with fermented oats},
  url          = {http://dx.doi.org/10.1016/S0168-1605(98)00055-5},
  volume       = {42},
  year         = {1998},
}