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Gastric bypass improves ss-cell function and increases β-cell mass in a porcine model.

Lindqvist, Andreas LU ; Spégel, Peter LU ; Ekelund, Mikael LU ; Garcia Vaz, Eliana LU ; Pierzynowski, Stefan; Gomez, Maria LU ; Mulder, Hindrik LU ; Hedenbro, Jan LU ; Groop, Leif LU and Wierup, Nils LU (2014) In Diabetes 63(5). p.1665-1671
Abstract
The most frequently used, and effective, treatment for morbid obesity is Roux-en-Y gastric bypass surgery (RYGB), which results in rapid remission of T2D in most cases. To what extent this is accounted for by weight loss or other factors remains elusive. To gain insight into these mechanisms, we investigated the effects of RYGB on ß-cell function and ß-cell mass in the pig, a species highly reminiscent of the human. RYGB was performed using linear staplers during open surgery. Sham-operated pigs were used as controls. Both groups were fed a low calorie diet for 3 weeks after surgery. Intravenous glucose-tolerance tests were performed 2 weeks after surgery. Body weight in RYGB-pigs and sham-operated, pair-fed control pigs developed... (More)
The most frequently used, and effective, treatment for morbid obesity is Roux-en-Y gastric bypass surgery (RYGB), which results in rapid remission of T2D in most cases. To what extent this is accounted for by weight loss or other factors remains elusive. To gain insight into these mechanisms, we investigated the effects of RYGB on ß-cell function and ß-cell mass in the pig, a species highly reminiscent of the human. RYGB was performed using linear staplers during open surgery. Sham-operated pigs were used as controls. Both groups were fed a low calorie diet for 3 weeks after surgery. Intravenous glucose-tolerance tests were performed 2 weeks after surgery. Body weight in RYGB-pigs and sham-operated, pair-fed control pigs developed similarly. RYGB-pigs displayed improved glycaemic control, which was attributed to increases in ß-cell mass, islet number and number of extra-islet ß-cells. Pancreatic expression of insulin and glucagon was elevated, and cells expressing the GLP-1-receptor were more abundant in RYGB-pigs. Our data from a pig model of RYGB emphasize the key role of improved ß-cell function and ß-cell mass to explain the improved glucose tolerance after RYGB as food intake and body weight remained identical. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Diabetes
volume
63
issue
5
pages
1665 - 1671
publisher
American Diabetes Association Inc.
external identifiers
  • pmid:24487021
  • wos:000334796600029
  • scopus:84899126845
ISSN
1939-327X
DOI
10.2337/db13-0969
language
English
LU publication?
yes
id
b1d2d5da-8519-404f-8b02-150fb1a4e95d (old id 4335894)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24487021?dopt=Abstract
date added to LUP
2014-03-07 20:38:35
date last changed
2017-10-22 03:01:07
@article{b1d2d5da-8519-404f-8b02-150fb1a4e95d,
  abstract     = {The most frequently used, and effective, treatment for morbid obesity is Roux-en-Y gastric bypass surgery (RYGB), which results in rapid remission of T2D in most cases. To what extent this is accounted for by weight loss or other factors remains elusive. To gain insight into these mechanisms, we investigated the effects of RYGB on ß-cell function and ß-cell mass in the pig, a species highly reminiscent of the human. RYGB was performed using linear staplers during open surgery. Sham-operated pigs were used as controls. Both groups were fed a low calorie diet for 3 weeks after surgery. Intravenous glucose-tolerance tests were performed 2 weeks after surgery. Body weight in RYGB-pigs and sham-operated, pair-fed control pigs developed similarly. RYGB-pigs displayed improved glycaemic control, which was attributed to increases in ß-cell mass, islet number and number of extra-islet ß-cells. Pancreatic expression of insulin and glucagon was elevated, and cells expressing the GLP-1-receptor were more abundant in RYGB-pigs. Our data from a pig model of RYGB emphasize the key role of improved ß-cell function and ß-cell mass to explain the improved glucose tolerance after RYGB as food intake and body weight remained identical.},
  author       = {Lindqvist, Andreas and Spégel, Peter and Ekelund, Mikael and Garcia Vaz, Eliana and Pierzynowski, Stefan and Gomez, Maria and Mulder, Hindrik and Hedenbro, Jan and Groop, Leif and Wierup, Nils},
  issn         = {1939-327X},
  language     = {eng},
  number       = {5},
  pages        = {1665--1671},
  publisher    = {American Diabetes Association Inc.},
  series       = {Diabetes},
  title        = {Gastric bypass improves ss-cell function and increases β-cell mass in a porcine model.},
  url          = {http://dx.doi.org/10.2337/db13-0969},
  volume       = {63},
  year         = {2014},
}