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Characteristics and functionality of appetite-reducing thylakoid powders produced by three different drying processes

Östbring, Karolina LU ; Sjöholm, Ingegerd LU ; Sörenson, Henrietta; Ekholm, Andrej; Erlanson-Albertsson, Charlotte LU and Rayner, Marilyn LU (2018) In Journal of the Science of Food and Agriculture 98(4). p.1554-1565
Abstract

BACKGROUND: Thylakoids, a chloroplast membrane extracted from green leaves, are a promising functional ingredient with appetite-reducing properties via their lipase-inhibiting effect. Thylakoids in powder form have been evaluated in animal and human models, but no comprehensive study has been conducted on powder characteristics. The aim was to investigate the effects of different isolation methods and drying techniques (drum-drying, spray-drying, freeze-drying) on thylakoids' physicochemical and functional properties. RESULTS: Freeze-drying yielded thylakoid powders with the highest lipase-inhibiting capacity. We hypothesize that the specific macromolecular structures involved in lipase inhibition were degraded to different degrees by... (More)

BACKGROUND: Thylakoids, a chloroplast membrane extracted from green leaves, are a promising functional ingredient with appetite-reducing properties via their lipase-inhibiting effect. Thylakoids in powder form have been evaluated in animal and human models, but no comprehensive study has been conducted on powder characteristics. The aim was to investigate the effects of different isolation methods and drying techniques (drum-drying, spray-drying, freeze-drying) on thylakoids' physicochemical and functional properties. RESULTS: Freeze-drying yielded thylakoid powders with the highest lipase-inhibiting capacity. We hypothesize that the specific macromolecular structures involved in lipase inhibition were degraded to different degrees by exposure to heat during spray-drying and drum-drying. We identified lightness (Hunter's L-value), greenness (Hunter's a-value), chlorophyll content and emulsifying capacity to be correlated to lipase-inhibiting capacity. Thus, to optimize the thylakoids functional properties, the internal membrane structure indicated by retained green colour should be preserved. This opens possibilities to use chlorophyll content as a marker for thylakoid functionality in screening processes during process optimization. CONCLUSION: Thylakoids are heat sensitive, and a mild drying technique should be used in industrial production. Strong links between physicochemical parameters and lipase inhibition capacity were found that can be used to predict functionality. The approach from this study can be applied towards production of standardized high-quality functional food ingredients.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
chloroplast membrane, drying, lipolysis, physicochemical properties, spinach
in
Journal of the Science of Food and Agriculture
volume
98
issue
4
pages
12 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:85041574211
ISSN
0022-5142
DOI
10.1002/jsfa.8627
language
English
LU publication?
yes
id
86d30fd0-f65e-4cc2-9595-eedb3c3c798e
date added to LUP
2018-02-20 12:07:06
date last changed
2018-05-29 11:47:12
@article{86d30fd0-f65e-4cc2-9595-eedb3c3c798e,
  abstract     = {<p>BACKGROUND: Thylakoids, a chloroplast membrane extracted from green leaves, are a promising functional ingredient with appetite-reducing properties via their lipase-inhibiting effect. Thylakoids in powder form have been evaluated in animal and human models, but no comprehensive study has been conducted on powder characteristics. The aim was to investigate the effects of different isolation methods and drying techniques (drum-drying, spray-drying, freeze-drying) on thylakoids' physicochemical and functional properties. RESULTS: Freeze-drying yielded thylakoid powders with the highest lipase-inhibiting capacity. We hypothesize that the specific macromolecular structures involved in lipase inhibition were degraded to different degrees by exposure to heat during spray-drying and drum-drying. We identified lightness (Hunter's L-value), greenness (Hunter's a-value), chlorophyll content and emulsifying capacity to be correlated to lipase-inhibiting capacity. Thus, to optimize the thylakoids functional properties, the internal membrane structure indicated by retained green colour should be preserved. This opens possibilities to use chlorophyll content as a marker for thylakoid functionality in screening processes during process optimization. CONCLUSION: Thylakoids are heat sensitive, and a mild drying technique should be used in industrial production. Strong links between physicochemical parameters and lipase inhibition capacity were found that can be used to predict functionality. The approach from this study can be applied towards production of standardized high-quality functional food ingredients.</p>},
  author       = {Östbring, Karolina and Sjöholm, Ingegerd and Sörenson, Henrietta and Ekholm, Andrej and Erlanson-Albertsson, Charlotte and Rayner, Marilyn},
  issn         = {0022-5142},
  keyword      = {chloroplast membrane,drying,lipolysis,physicochemical properties,spinach},
  language     = {eng},
  month        = {03},
  number       = {4},
  pages        = {1554--1565},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of the Science of Food and Agriculture},
  title        = {Characteristics and functionality of appetite-reducing thylakoid powders produced by three different drying processes},
  url          = {http://dx.doi.org/10.1002/jsfa.8627},
  volume       = {98},
  year         = {2018},
}