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Comparative immunomodulatory effects in mice and in human dendritic cells of five bacterial strains selected for biocontrol of leafy green vegetables

Uhlig, Elisabeth LU ; Elli, Giulia LU ; Nurminen, Noora ; Oscarsson, Elin LU ; Canaviri-Paz, Pamela LU ; Burri, Stina LU ; Rohrstock, Anne-Marie LU ; Rahman, Milladur LU orcid ; Alsanius, Beatrix and Molin, Göran LU , et al. (2022) In Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 165.
Abstract

The market for ready-to eat vegetables is increasing, but unfortunately so do the numbers of food-borne illness outbreaks related to these products. A previous study has identified bacterial strains suitable for biocontrol of leafy green vegetables to reduce the exposure to pathogens in these products. As a tentative safety evaluation, five selected strains (Rhodococcus cerastii MR5x, Bacillus coagulans LMG P-32205, Bacillus coagulans LMG P-32206, Pseudomonas cedrina LMG P-32207 and Pseudomonas punonensis LMG P-32204) were individually compared for immunomodulating effects in mice and in human monocyte-derived dendritic cells (MoDCs). Mice receiving the two B. coagulans strains consistently resemble the immunological response of the... (More)

The market for ready-to eat vegetables is increasing, but unfortunately so do the numbers of food-borne illness outbreaks related to these products. A previous study has identified bacterial strains suitable for biocontrol of leafy green vegetables to reduce the exposure to pathogens in these products. As a tentative safety evaluation, five selected strains (Rhodococcus cerastii MR5x, Bacillus coagulans LMG P-32205, Bacillus coagulans LMG P-32206, Pseudomonas cedrina LMG P-32207 and Pseudomonas punonensis LMG P-32204) were individually compared for immunomodulating effects in mice and in human monocyte-derived dendritic cells (MoDCs). Mice receiving the two B. coagulans strains consistently resemble the immunological response of the normal control, and no, or low, cell activation and pro-inflammatory cytokine expression was observed in MoDCs exposed to B. coagulans strains. However, different responses were seen in the two models for the Gram-negative P. cedrina and the Gram-positive R. cerastii. Moreover, P. punonensis and B. coagulans increased the microbiota diversity in mice as seen by the Shannon-Wiener index. In conclusion, the two strains of B. coagulans showed an immunological response that indicate that they lack pathogenic abilities, thus encouraging further safety evaluation and showing great potential to be used as biocontrol agents on leafy green vegetables.

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Contribution to journal
publication status
published
subject
in
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
volume
165
article number
113064
publisher
Elsevier
external identifiers
  • pmid:35561874
  • scopus:85130166185
ISSN
1873-6351
DOI
10.1016/j.fct.2022.113064
language
English
LU publication?
yes
additional info
Copyright © 2022. Published by Elsevier Ltd.
id
89288a21-ee57-4540-b6fa-add88e5c529f
date added to LUP
2022-05-18 08:42:47
date last changed
2024-07-25 21:49:55
@article{89288a21-ee57-4540-b6fa-add88e5c529f,
  abstract     = {{<p>The market for ready-to eat vegetables is increasing, but unfortunately so do the numbers of food-borne illness outbreaks related to these products. A previous study has identified bacterial strains suitable for biocontrol of leafy green vegetables to reduce the exposure to pathogens in these products. As a tentative safety evaluation, five selected strains (Rhodococcus cerastii MR5x, Bacillus coagulans LMG P-32205, Bacillus coagulans LMG P-32206, Pseudomonas cedrina LMG P-32207 and Pseudomonas punonensis LMG P-32204) were individually compared for immunomodulating effects in mice and in human monocyte-derived dendritic cells (MoDCs). Mice receiving the two B. coagulans strains consistently resemble the immunological response of the normal control, and no, or low, cell activation and pro-inflammatory cytokine expression was observed in MoDCs exposed to B. coagulans strains. However, different responses were seen in the two models for the Gram-negative P. cedrina and the Gram-positive R. cerastii. Moreover, P. punonensis and B. coagulans increased the microbiota diversity in mice as seen by the Shannon-Wiener index. In conclusion, the two strains of B. coagulans showed an immunological response that indicate that they lack pathogenic abilities, thus encouraging further safety evaluation and showing great potential to be used as biocontrol agents on leafy green vegetables.</p>}},
  author       = {{Uhlig, Elisabeth and Elli, Giulia and Nurminen, Noora and Oscarsson, Elin and Canaviri-Paz, Pamela and Burri, Stina and Rohrstock, Anne-Marie and Rahman, Milladur and Alsanius, Beatrix and Molin, Göran and Zeller, Kathrin Stephanie and Håkansson, Åsa}},
  issn         = {{1873-6351}},
  language     = {{eng}},
  month        = {{05}},
  publisher    = {{Elsevier}},
  series       = {{Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association}},
  title        = {{Comparative immunomodulatory effects in mice and in human dendritic cells of five bacterial strains selected for biocontrol of leafy green vegetables}},
  url          = {{http://dx.doi.org/10.1016/j.fct.2022.113064}},
  doi          = {{10.1016/j.fct.2022.113064}},
  volume       = {{165}},
  year         = {{2022}},
}