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The effect of heat treatment of thylakoids on their ability to inhibit in vitro lipase/co-lipase activity.

Östbring, Karolina LU ; Rayner, Marilyn LU ; Sjöholm, Ingegerd LU ; Otterström, Jennie; Albertsson, Per-Åke LU ; Emek, Sinan Cem LU and Erlanson-Albertsson, Charlotte LU (2014) In Food & Function 5(9). p.2157-2165
Abstract
Thylakoids has been shown to prolong lipolysis by the inhibition of lipase/co-lipase, which makes thylakoids suitable as a functional food ingredient with satiating properties. The components of thylakoids that provide its function as a lipolysis modulator are primarily photosystems I and II, which are structurally stabilised by chlorophyll. However, chlorophyll is known to be heat sensitive yet the enzymatic inhibiting capacity after heat treatment has not been previously studied. It was hypothesised that the retained function of thylakoids after heat treatment could be correlated to the degree of degradation. Heat treatment at either 60 °C, 75 °C or 90 °C for time interval ranging from 15 s to 120 min induced a color shift from bright... (More)
Thylakoids has been shown to prolong lipolysis by the inhibition of lipase/co-lipase, which makes thylakoids suitable as a functional food ingredient with satiating properties. The components of thylakoids that provide its function as a lipolysis modulator are primarily photosystems I and II, which are structurally stabilised by chlorophyll. However, chlorophyll is known to be heat sensitive yet the enzymatic inhibiting capacity after heat treatment has not been previously studied. It was hypothesised that the retained function of thylakoids after heat treatment could be correlated to the degree of degradation. Heat treatment at either 60 °C, 75 °C or 90 °C for time interval ranging from 15 s to 120 min induced a color shift from bright green to olive brown which was attributed to degradation. The ability of heat-treated thylakoids to inhibit lipolysis in vitro was also reduced. A correlation between chlorophyll a degradation and the enzymatic inhibiting capacity could be established which opens possibilities to use a spectrophotometric method to quantify the ability of thylakoids to inhibit lipase/co-lipase in a more rapid and cost effective way to complement the pH-stat method used today. With the degradation pattern investigated, it is then possible to design a thermal treatment process to ensure a microbiological safe appetite-reducing product and at the same time minimize the loss of functionality. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Food & Function
volume
5
issue
9
pages
2157 - 2165
publisher
Royal Society of Chemistry
external identifiers
  • pmid:25016966
  • wos:000341016500019
  • scopus:84906549423
ISSN
2042-6496
DOI
10.1039/c3fo60651a
language
English
LU publication?
yes
id
f3e58454-c012-4e68-b405-6f988c20fe35 (old id 4582431)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/25016966?dopt=Abstract
date added to LUP
2014-08-07 22:29:53
date last changed
2017-06-20 11:07:10
@article{f3e58454-c012-4e68-b405-6f988c20fe35,
  abstract     = {Thylakoids has been shown to prolong lipolysis by the inhibition of lipase/co-lipase, which makes thylakoids suitable as a functional food ingredient with satiating properties. The components of thylakoids that provide its function as a lipolysis modulator are primarily photosystems I and II, which are structurally stabilised by chlorophyll. However, chlorophyll is known to be heat sensitive yet the enzymatic inhibiting capacity after heat treatment has not been previously studied. It was hypothesised that the retained function of thylakoids after heat treatment could be correlated to the degree of degradation. Heat treatment at either 60 °C, 75 °C or 90 °C for time interval ranging from 15 s to 120 min induced a color shift from bright green to olive brown which was attributed to degradation. The ability of heat-treated thylakoids to inhibit lipolysis in vitro was also reduced. A correlation between chlorophyll a degradation and the enzymatic inhibiting capacity could be established which opens possibilities to use a spectrophotometric method to quantify the ability of thylakoids to inhibit lipase/co-lipase in a more rapid and cost effective way to complement the pH-stat method used today. With the degradation pattern investigated, it is then possible to design a thermal treatment process to ensure a microbiological safe appetite-reducing product and at the same time minimize the loss of functionality.},
  author       = {Östbring, Karolina and Rayner, Marilyn and Sjöholm, Ingegerd and Otterström, Jennie and Albertsson, Per-Åke and Emek, Sinan Cem and Erlanson-Albertsson, Charlotte},
  issn         = {2042-6496},
  language     = {eng},
  number       = {9},
  pages        = {2157--2165},
  publisher    = {Royal Society of Chemistry},
  series       = {Food & Function},
  title        = {The effect of heat treatment of thylakoids on their ability to inhibit in vitro lipase/co-lipase activity.},
  url          = {http://dx.doi.org/10.1039/c3fo60651a},
  volume       = {5},
  year         = {2014},
}